VTCN1 antibody (Internal Region)

Catalog No. ABIN334524

The Goat Polyclonal anti-VTCN1 antibody (ABIN334524) specifically detects VTCN1 in WB, FACS, ELISA and IF. The antibody is reactive with Human samples.

Quick Overview for VTCN1 antibody (Internal Region) (ABIN334524)

Target: VTCN1 (V-Set Domain Containing T Cell Activation Inhibitor 1 (VTCN1))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This VTCN1 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), ELISA, Immunofluorescence (IF)

Grade: Verified

Product Details anti-VTCN1 Antibody

Binding Specificity: Internal Region

Purpose: B7-H4

Sequence: SKGKGNANLE YK

Cross-Reactivity: Dog, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-SKGKGNANLEYK, from the internal region of the protein sequence according to NP_078902.2.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 26+21 kDa bands observed in HEK293 cell lysates, which were both successfully blocked by incubation with the immunizing peptide. An approx 26 kDa and 21 kDa band observed in Human Adrenal Gland lysates, and approx 27 kDa in Human Pancrea

Peptide ELISA: antibody detection limit dilution 1:64000.

Comment:

Immunofluorescence: Strong expression of the protein seen in the cytoplasm and membranes/cell junctions of MCF7 cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of MCF7 cells. Recomme

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for VTCN1

Target: VTCN1 (V-Set Domain Containing T Cell Activation Inhibitor 1 (VTCN1))

Alternative Name: VTCN1

Background: B7-H4, B7H4, B7S1, B7X, B7h.5, FLJ22418, PRO1291, RP11-229A19.4, VCTN1, T cell costimulatory molecule B7x, immune costimulatory protein, V-set domain containing T cell activation inhibitor 1

Molecular Weight: 30.9kDa

Gene ID: 79679, 242122, 295322

NCBI Accession: NP_078902, NP_001240778