V-Set Domain Containing T Cell Activation Inhibitor 1 (VTCN1) antibody

Antigen: V-Set Domain Containing T Cell Activation Inhibitor 1 (VTCN1)

Synonyms: B7X, B7H4, B7S1, B7-H4, B7h.5, VCTN1, PRO1291, FLJ22418, RP11-229A19.4, B7x, B7h4, B7s1, BC032925, MGC41287, RGD1311204, MGC52801, MGC89259

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN334524

Additional Information

Alternative name: B7-H4

Gene ID: 79679, 242122 (mouse), 295322 (rat)

Swiss-Prot: NP_078902.2

Immunogen: Peptide with sequence C-SKGKGNANLEYK, from the internal region of the protein sequence according to NP_078902.2.

Cross-Reactivity: Human, Mouse (Murine), Rat (Rattus), Dog (Canine)

Format: Liquid

Description: Principal Names: V-set domain containing T cell activation inhibitor 1, immune costimulatory protein, T cell costimulatory molecule B7x, VCTN1, RP11-229A19.4, PRO1291, FLJ22418, B7h.5, B7X, B7S1, B7H4, B7-H4. Official Symbol: VTCN1

Specificity: Human, Mouse, Rat, Dog

Molecular Weight: 30.9kDa

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:64000. Western blot: Approx 28kDa band observed in Human Pancrease lysates (calculated MW of 30.9kDa according to NP_078902.2). Recommended concentration: 0.5-1.5µg/ml.

Concentration: 100µg/200µl

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Images

ABIN334524 (0.3µg/ml) staining of Human Spleen lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

HEK293 overexpressing B7-H4 (RC210360) and probed with ABIN334524 (mock transfection in first lane), tested by Origene.

Publications

Publications: Krambeck, Thompson, Dong et al.: "B7-H4 expression in renal cell carcinoma and tumor vasculature: associations with cancer progression and survival." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 103, Issue 27, pp. 10391-6, 2006 (PubMed).