»
Antibodies
»
anti-D1 Protein of PSII, C-terminal (PsbA) antibody
D1 Protein of PSII, C-terminal (PsbA) antibody
| Antigen |
|
| Clonality |
Polyclonal |
|
Host
|
|
|
Reactivity
|
 Alternatives Arabidopsis thaliana, Barley (Hordeum vulgare), Proso millet (Panicum miliaceum), Guinea gras (Panicum maximum), Maize/Corn (Zea mays), Moss (Physcomitrella patens), Chlamydomonas reinhardtii (C. reinhardtii), Synechococcus PCC 7942, Cyanobacterium Anabaena sp. PCC 7120, Prochlorococcus, Zooxanthellae (Symbiodinium)
Arabidopsis thaliana (3), Barley (Hordeum vulgare) (3), Chlamydomonas reinhardtii (C. reinhardtii) (3), Synechococcus PCC 7942 (3), Amphidinium carterae (A. carterae) (2), Chlamydomonas raudensis (2), Cyanophora (2), Fucus vesiculosus (2), Gonyaulax polyedra (2), Lobaria pulmonaria (2), Petunia (2), Pinus sylvestris (2), Seaweed (Alaria esculenta) (2), Ulva (2), Cyanobacterium Anabaena sp. PCC 7120 (1), Guinea gras (Panicum maximum) (1), Maize/Corn (Zea mays) (1), Moss (Physcomitrella patens) (1), Pea (Pisum sativum) (1), Prochlorococcus (1), Proso millet (Panicum miliaceum) (1), Zooxanthellae (Symbiodinium) (1)
|
|
Application
|
|
| Catalog no. |
ABIN334536 |
| Quantity |
10 ul (Variants) |
| Price |
247.00 $ Plus shipping costs $35.00
|
| Shipping to |
|
| Availability |
Ships within 3 to 5 Business Days |
|
Immunogen
|
KLH-conjugated synthetic peptide derived from available plant,algal andcyanobacterial PsbA sequences, including Arabidopsis thaliana AtCg00020,Oryza sativa P0C434, Physcomitrella patens Q6YXN7, Chlamydomonasreinhardtii P07753, Synechococcus sp. P14660
|
|
Format
|
Lyophilized
|
|
Description
|
The psbA gene has been cloned from many species of plants, green algae, andcyanobacteria. The psbA gene is located in the chloroplast genome and encodesfor the D1 protein, a core component of Photosystem II. PsbA/D1 is rapidly cycledunder illumination in all oxygenic photobionts. Tracking PsbA pools using theGlobal PsbA antibody can show the functional content of Photosystem II in a widerange of samples.
|
|
Specificity
|
Predicted reactivity: di and monocots,conifers, brown and red algae, cyanobacteria, cellular[compartment marker] of thylakoid membrane.
|
|
Application Notes
|
Recommended Dilution 1: 10 000 with standard ECL (WB). Additional Information: A number of degradation products may be observed when using anti-PsbAantibodies, including products having apparent molecular weights of 24kDa and16kDa. D1 degradation is a complex set of events and the products observed canbe influenced by both the extraction procedure and the physiology of the cellsprior to harvest. Third, cross-linking may occur between D1 and cytochrome b559,shifting the protein higher in the gel. In cyanobacteria (PCC7942), three differentbands were competed out by preincubating the antibody with the PsbA freepeptide, indicating that all bands are indeed PsbA and its precursors orbreakdown product.
|
|
Purity
|
Serum
|
|
Storage
|
store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior toopening them to avoid any losses that might occur from lyophilized materialadhering to the cap or sides of the tubes.
|
|
Research Area
|
Plant/Algal Physiology
|
|
Restrictions
|
For Research Use only
|
|
Publications
|
Brown, Campbell, Lawrence: "Resource dynamics during infection of Micromonas pusilla by virus MpV-Sp1." in: Environmental microbiology, Vol. 9, Issue 11, pp. 2720-7, 2007 (PubMed).
|
Alternatives for antigen "D1 Protein of PSII, C-terminal (PsbA)", type "Antibodies"
|
Hosts
|
Chicken (2), Rabbit (1)
|
|
Reactivities
|
Arabidopsis thaliana (3), Barley (Hordeum vulgare) (3), Chlamydomonas reinhardtii (C. reinhardtii) (3), Synechococcus PCC 7942 (3), Amphidinium carterae (A. carterae) (2), Chlamydomonas raudensis (2), Cyanophora (2), Fucus vesiculosus (2), Gonyaulax polyedra (2), Lobaria pulmonaria (2), Petunia (2), Pinus sylvestris (2), Seaweed (Alaria esculenta) (2), Ulva (2), Cyanobacterium Anabaena sp. PCC 7120 (1), Guinea gras (Panicum maximum) (1), Maize/Corn (Zea mays) (1), Moss (Physcomitrella patens) (1), Pea (Pisum sativum) (1), Prochlorococcus (1), Proso millet (Panicum miliaceum) (1), Zooxanthellae (Symbiodinium) (1)
|
|
Applications
|
Western Blotting (WB) (3)
|
