Keratin 14 (KRT14) antibody

Antigen: Keratin 14 (KRT14)

Synonyms: K14, NFJ, CK14, EBS3, EBS4, Krt1-14, AI626930, Krt-1.14, Krt14l, Krt14, Ka14, KRT14, ouro2

Clonality: Monoclonal (RCK107)

Host: Mouse

Reactivity: Human, Rat (Rattus), Dog (Canine), Pig (Porcine)

Application: Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB)

Catalog no.: ABIN335329

Additional Information

Alternative name: Cytokeratin 14 / Keratin K14

Immunogen: RCK107 is a mouse monoclonal IgG3 antibody derived by fusion of SP2/0-Ag14 mouse myeloma cells with spleen cells from a mouse immunized with a cytoskeletal preparation of TR146 epithelial cells.

Format: Purified

Isotype: IgG1  (Matching secondary antibodies)

Clone: RCK107

Description: Cytokeratins are a subfamily of intermediate filament proteins and are characterized by a remarkable biochemical diversity, represented in human epithelial tissues by at least 20 different polypeptides. They range in molecular weight between 40 kDa and 68 kDa and isoelectric pH between 4.9 - 7.8. The individual human cytokeratins are numbered 1 to 20. The various epithelia in the human body usually express cytokeratins which are not only characteristic of the type of epithelium, but also related to the degree of maturation or differentiation within an epithelium. Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies. The cytokeratin antibodies are not only of assistance in the differential diagnosis of tumors using immunohistochemistry on tissue sections, but are also a useful tool in cytopathology and flow cytometric assays.

Specificity: Human, rat, canine and swine.

Application Details

Application Notes: RCK107 reacts exclusively with cytokeratin 14 which is present in basal cell compartments of stratified and combined epithelia. RCK107 is suitable for immunocytochemistry, immunohistochemistry on frozen sections, flow cytometry and immunoblotting. Optimal antibody dilution should be determined by titration, recommended range is 1:100 - 1:200 for flow cytometry, and for immunohistochemistry with avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent, and 1:100 - 1:1000 for immunoblotting applications.

Storage: Store at 4 o C, or in small aliquots at -20 o C.

Restrictions: For Research Use only

Images

anti-Keratin 14 (KRT14) antibody

anti-Keratin 14 (KRT14) antibody (Image 2)

Publications

Publications: Vos, van den Ingh, de Neijs et al.: "Immunohistochemistry with keratin monoclonal antibodies in canine tissues: urogenital tract, respiratory tract, (neuro-)endocrine tissues, choroid plexus and spinal cord." in: Zentralblatt für Veterinärmedizin. Reihe A, Vol. 39, Issue 10, pp. 721-40, 1993 (PubMed).

Smedts, Ramaekers, Troyanovsky et al.: "Basal-cell keratins in cervical reserve cells and a comparison to their expression in cervical intraepithelial neoplasia." in: The American journal of pathology, Vol. 140, Issue 3, pp. 601-12, 1992 (PubMed).

Wetzels, Schaafsma, Leigh et al.: "Laminin and type VII collagen distribution in different types of human lung carcinoma: correlation with expression of keratins 14, 16, 17 and 18." in: Histopathology, Vol. 20, Issue 4, pp. 295-303, 1992 (PubMed).

Bauwens, De Groot, Ramaekers et al.: "Expression of intermediate filament proteins in the adult human vestibular labyrinth." in: The Annals of otology, rhinology, and laryngology, Vol. 101, Issue 6, pp. 479-86, 1992 (PubMed).

Smedts, Ramaekers, Troyanovsky et al.: "Keratin expression in cervical cancer." in: The American journal of pathology, Vol. 141, Issue 2, pp. 497-511, 1992 (PubMed).

Wetzels, Kuijpers, Lane et al.: "Basal cell-specific and hyperproliferation-related keratins in human breast cancer." in: The American journal of pathology, Vol. 138, Issue 3, pp. 751-63, 1991 (PubMed).

Meek, Van Elssen, Huijskens et al.: "T cells fail to develop in the human skin-cell explants system; an inconvenient truth." in: BMC immunology, Vol. 12, Issue 1, pp. 17, 2011 (PubMed).