Keratin 19 (KRT19) antibody

Antigen: Keratin 19 (KRT19)

Synonyms: K19, CK19, K1CS, MGC15366, EndoC, Krt1-19, AI663979, Krt-1.19, MGC25344, Ka19, MGC124534, MGC156553, k19, ck19, k1cs, krt9, krt15, MGC76282, KRT19, GK-19, MGC83069, LOC514812, MGC154386, DKFZp469N0613

Clonality: Monoclonal (RCK108)

Host: Mouse

Reactivity: Human

Application: Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)

Catalog no.: ABIN335332

Additional Information

Alternative name: Cytokeratin 19 / Keratin K19

Immunogen: RCK108 is a mouse monoclonal IgG1 antibody derived by fusion of SP2/0-Ag14 mouse myeloma cells with spleen cells from a BALB/c mouse immunized with a cytoskeletal preparation of human bladder carcinoma cell line T24.

Format: Purified

Isotype: IgG1  (Matching secondary antibodies)

Clone: RCK108

Description: Cytokeratins are a subfamily of intermediate filament proteins and are characterized by a remarkable biochemical diversity, represented in human epithelial tissues by at least 20 different polypeptides. They range in molecular weight between 40 kDa and 68 kDa and isoelectric pH between 4.9 - 7.8. The individual human cytokeratins are numbered 1 to 20. The various epithelia in the human body usually express cytokeratins which are not only characteristic of the type of epithelium, but also related to the degree of maturation or differentiation within an epithelium. Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies. The cytokeratin antibodies are not only of assistance in the differential diagnosis of tumors using immunohistochemistry on tissue sections, but are also a useful tool in cytopathology and flow cytometric assays.

Specificity: Human.

Application Details

Application Notes: RCK108 reacts exclusively with cytokeratin 19 which is present in glandular-type epithelia and most carcinomas. Does not react with hepatocytes and hepatocellular carcinoma. RCK108 is particularly well suited for use in immunohistochemistry on paraffin-embedded tissues after pretreatment with pepsin or trypsin. RCK108 is also useful for immunocytochemistry, immunohistochemistry on frozen sections, immunoblotting and flow cytometry. Optimal antibody dilution should be determined by titration, recommended range is 1:100 - 1:200 for flow cytometry, and for immunohistochemistry with avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent, and 1:100 - 1:1000 for immunoblotting applications.

Storage: Store at 4 o C, or in small aliquots at -20 o C.

Restrictions: For Research Use only

Images

anti-Keratin 19 (KRT19) antibody

anti-Keratin 19 (KRT19) antibody (Image 2)

Publications

Publications: Kwaspen, Smedts, Broos et al.: "Reproducible and highly sensitive detection of the broad spectrum epithelial marker keratin 19 in routine cancer diagnosis." in: Histopathology, Vol. 31, Issue 6, pp. 503-16, 1998 (PubMed).