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anti-Cytokeratin 17 / Keratin K17 antibody

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Image for anti-Cytokeratin 17 / Keratin K17 antibody
Antigen: Cytokeratin 17 / Keratin K17
Clonality: Monoclonal (E3)
Application: Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
Reactivity: Human, Rat (Rattus)
Host: Mouse
7 references available 7 references available
Quantity: 100ug
Price: 351,79 $   (Plus shipping costs $25 and VAT, Invoice in EUR)
Order number: ABIN335340
Availability: Ships within 1 to 3 Business days
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Additional Information  » anti-Cytokeratin 17 / Keratin K17 antibody

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Antigen Cytokeratin 17 / Keratin K17
Immunogen E3 is a mouse monoclonal IgG2b antibody derived by fusion of X63 Ag 8.653 mouse myeloma cells with spleen cells from a Balb/c mouse immunized with a cytoskeletal preparation from rat colon.
Reactivity Human, Rat (Rattus)
Clonality Monoclonal
Format Purified
Isotype IgG2b »Matching secondary antibodies
Description Cytokeratins are a subfamily of intermediate filament proteins and are characterized by a remarkable biochemical diversity, represented in human epithelial tissues by at least 20 different polypeptides. They range in molecular weight between 40 kDa and 68 kDa and isoelectric pH between 4.9 - 7.8. The individual human cytokeratins are numbered 1 to 20. The various epithelia in the human body usually express cytokeratins which are not only characteristic of the type of epithelium, but also related to the degree of maturation or differentiation within an epithelium. Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies. The cytokeratin antibodies are not only of assistance in the differential diagnosis of tumors using immunohistochemistry on tissue sections, but are also a useful tool in cytopathology and flow cytometric assays.
Clone E3
Host Mouse
Specificity Human and rat.

Application Details  » anti-Cytokeratin 17 / Keratin K17 antibody

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Application Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
» Click here to view 8 available protocols!
Application Notes E3 reacts with cytokeratin 17 in basal layers of pseudo-stratified and transitional epithelia. APPLICATIONS E3 is suitable for immunoblotting, immunocytochemistry, immunohistochemistry on frozen and paraffin-embedded tissues and flow cytometry. Optimal antibody dilution should be determined by titration, recommended range is 1:25 - 1:200 for flow cytometry, and for immunohistochemistry with avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent, and 1:100 - 1:1000 for immunoblotting applications.
Storage Store at 4 o C, or in small aliquots at -20 o C.
Research Area Extracellular Matrix
Restrictions For Research Use only

Publications  » anti-Cytokeratin 17 / Keratin K17 antibody

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Publications Smedts, Ramaekers, Troyanovsky et al.: "Basal-cell keratins in cervical reserve cells and a comparison to their expression in cervical intraepithelial neoplasia." In: The American journal of pathology , Vol. 140, Issue 3, pp. 601-12, 1992 (PubMed)

Wetzels, Schaafsma, Leigh et al.: "Laminin and type VII collagen distribution in different types of human lung carcinoma: correlation with expression of keratins 14, 16, 17 and 18." In: Histopathology , Vol. 20, Issue 4, pp. 295-303, 1992 (PubMed)

Smedts, Ramaekers, Troyanovsky et al.: "Keratin expression in cervical cancer." In: The American journal of pathology , Vol. 141, Issue 2, pp. 497-511, 1992 (PubMed)

Wetzels, Kuijpers, Lane et al.: "Basal cell-specific and hyperproliferation-related keratins in human breast cancer." In: The American journal of pathology , Vol. 138, Issue 3, pp. 751-63, 1991 (PubMed)

Guelstein, Tchypysheva, Ermilova et al.: "Monoclonal antibody mapping of keratins 8 and 17 and of vimentin in normal human mammary gland, benign tumors, dysplasias and breast cancer." In: International journal of cancer. Journal international du cancer , Vol. 42, Issue 2, pp. 147-53, 1988 (PubMed)

Troyanovsky, Guelstein, Tchipysheva et al.: "Patterns of expression of keratin 17 in human epithelia: dependency on cell position." In: Journal of cell science , Vol. 93 ( Pt 3), pp. 419-26, 1990 (PubMed)

Smedts, Ramaekers, Link et al.: "Detection of keratin subtypes in routinely processed cervical tissue: implications for tumour classification and the study of cervix cancer aetiology." In: Virchows Archiv : an international journal of pathology , Vol. 425, Issue 2, pp. 145-55, 1994 (PubMed)