Smoothelin (SMTN) antibody

Antigen: Smoothelin (SMTN)

Synonyms: FLJ35365, FLJ38597, smsmo, MGC68765, SMTN, MGC140000, MGC155237, DKFZp468D019

Clonality: Monoclonal (R4A)

Host: Mouse

Reactivity: Human, Dog (Canine), Cat (Feline), Chicken, Pig (Porcine), Monkey

Conjugate: Un-conjugated

Application: Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)

Catalog no.: ABIN335397

Additional Information

Alternative name: Smoothelin

Immunogen: R4A is a mouse monoclonal IgG1 antibody derived by fusion of SP2/0-Ag14 mouse myeloma cells with spleen cells from a mouse immunized with a cytoskeletal extract of chicken gizzard.

Isotype: IgG1

Clone: R4A

Description: Smoothelin is a constituent of the smooth muscle cell (SMC) cytoskeleton. Antibodies directed to smoothelin are useful tools to monitor SMC differentiation. Smoothelin is exclusively expressed in fully differentiated (contractile) SMCs. RNA and protein analyses revealed a broad species distribution of this protein. Cells with SMC-like characteristics, such as myofibroblasts and myoepithelial cells, as well as skeletal and cardiac muscle do not contain smoothelin. Confocal scanning laser microscopy of tissue sections and cells in culture show a filamentous organization of smoothelin colocalizing with actin stress fibers. In immunoblots two molecular weight isoforms are detected i.e. a 59 kDa isoform specific for visceral SMC (smoothelin A), and an isoform with a molecular weight of approximately 100 kDa in vascular SMC (smoothelin B). Human smoothelin is encoded by a single copy gene which is located on chromosome 22.

Specificity: Human, canine, feline, chicken, swine, monkey. Not reactive with rat.

Application Details

Application Notes: R4A reacts with the 59 kDa and 100 kDa protein, corresponding to smoothelin A and B, respectively, which are exclusively found in smooth muscle cells. R4A is suitable for immunoblotting and immunohistochemistry on frozen and paraffin-embedded tissues. For staining paraffin-embedded tissues pretreatment in 10 mM citrate buffer (pH 6.0) and heating for 3 times 5 minutes in a microwave is required. Optimal antibody dilution should be determined by titration, recommended range is 1:100 - 1:200 for immunohistochemistry with avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent, and 1:100 - 1:1000 for immunoblotting applications.

Concentration: 1mg/ml

Purification: Purified

Buffer: in PBS containing 0.09% Sodium Azide

Storage: Store at 4 o C, or in small aliquots at -20 o C.

Research Area: Organogenesis, Cytoskeleton

Restrictions: For Research Use only

Images

anti-Smoothelin (SMTN) antibody

anti-Smoothelin (SMTN) antibody (Image 2)

Publications

van der Loop, Schaart, Timmer et al.: "Smoothelin, a novel cytoskeletal protein specific for smooth muscle cells." in: The Journal of cell biology, Vol. 134, Issue 2, pp. 401-11, 1996 (PubMed).

Wehrens, Mies, Gimona et al.: "Localization of smoothelin in avian smooth muscle and identification of a vascular-specific isoform." in: FEBS letters, Vol. 405, Issue 3, pp. 315-20, 1997 (PubMed).

van der Loop, Gabbiani, Kohnen et al.: "Differentiation of smooth muscle cells in human blood vessels as defined by smoothelin, a novel marker for the contractile phenotype." in: Arteriosclerosis, thrombosis, and vascular biology, Vol. 17, Issue 4, pp. 665-71, 1997 (PubMed).

Christen, Bochaton-Piallat, Neuville et al.: "Cultured porcine coronary artery smooth muscle cells. A new model with advanced differentiation." in: Circulation research, Vol. 85, Issue 1, pp. 99-107, 1999 (PubMed).

Johansson, Eriksson, Ramaekers et al.: "Smoothelin in adult and developing human arteries and myocardium." in: Histochemistry and cell biology, Vol. 112, Issue 4, pp. 291-9, 2000 (PubMed).

Johansson, Eriksson, Ramaekers et al.: "Smoothelin and intermediate filament proteins in human aortocoronary saphenous vein by-pass grafts." in: The Histochemical journal, Vol. 31, Issue 11, pp. 723-7, 2000 (PubMed).

Deruiter, Rensen, Coolen et al.: "Smoothelin expression during chicken embryogenesis: detection of an embryonic isoform." in: Developmental dynamics : an official publication of the American Association of Anatomists, Vol. 221, Issue 4, pp. 460-3, 2001 (PubMed).