Recombinant Smoothelin antibody

Catalog No. ABIN7862098

The Rabbit Monoclonal anti-Smoothelin antibody (Clone SMTN-2320R) (ABIN7862098) specifically detects Smoothelin in IHC. The antibody is reactive with Human samples.

Quick Overview for Recombinant Smoothelin antibody (ABIN7862098)

Target: Smoothelin (SMTN)

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This Smoothelin antibody is un-conjugated

Application: Immunohistochemistry (IHC)

Clone: SMTN-2320R

Product Details anti-Smoothelin Antibody

Purpose: Recombinant Smoothelin (Differentiated Smooth Muscle Marker) Antibody

Specificity: Smoothelin is found exclusively in contractile smooth muscle cells. It associates with stress fibers and constitutes part of the cytoskeleton. In the vascular system, smoothelin expression is limited to large veins and arteries capable of pulsatile contraction. As a marker for the highly differentiated contractile smooth muscle cell, smoothelin expression is useful for studying vascular malformation and injury. It is a constituent of the smooth muscle cell cytoskeleton protein exclusively found in differentiated smooth muscle cells (SMC). Cells with SMC-like characteristics, such as myofibroblasts and myoepithelial cells, as well as skeletal and cardiac muscle do not contain smoothelin.To distinguish bladder muscularis mucosae (MM) from muscularis propria (MP) muscle bundles is crucial for accurate staging of bladder carcinoma. Strong smoothelin expression is nearly exclusively observed in muscularis propria. Therefore, the staining pattern of MP (strongly positive) and MM (negative or weakly positive) makes this antibody an attractive diagnostic tool. Anti-smoothelin immunostaining can be helpful in differentiating benign (+) from malignant smooth muscle tumors (-), and other mimics (-).

Purification: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant full-length human Smoothelin (SMTN) protein

Isotype: IgG, kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10  mM PBS

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for Smoothelin

Target: Smoothelin (SMTN)

Alternative Name: Smoothelin

Background:

FLJ35168, FLJ35365, FLJ35620, FLJ38597, Smoothelin, SMSMO, Smtn, SMTN_HUMAN

Human smooth muscle.

Molecular Weight: 59/110kDa (short/long isoforms|respectively)

Gene ID: 6525, 149098

UniProt: P53814