8-Hydroxyguanosine (8-OHDG) antibody

Antigen: 8-Hydroxyguanosine (8-OHDG)

Clonality: Monoclonal (N45-1)

Host: Mouse

Application: Immunohistochemistry (IHC), ELISA

Catalog no.: ABIN335419

Additional Information

Alternative name: 8 Hydroxyguanosine (8-OHDG)

Immunogen: 8-OHdG-conjugated Keyhole Limpet Hemocyanin

Format: Lyophilized

Isotype: IgG1 kappa

Clone: N45-1

Description: 8-hydroxy-2'-deoxyguanosine (8-OHdG) is a product of oxidatively damaged DNA formed by hydroxy radical, singlet oxygen and direct photodynamic action. Anti 8-OHdG monoclonal antibody (clone N45.1) is highly specific for 8-OHdG, and is suitable for immunohistochemistry. Cross reactivity have been checked for 8-OHdG analogues (guanosine(G),7-methyl-G, 6-SH-G, 8-Bromo-G, dA, dC, dT, dI, dU, dG, O6-methyl-dG, 8-OHdA, guanine(Gua),O6-methyl-Gua, 8-OH-Gua, uric acid, urea, creatine, creatinine, 8-sulfhydryl-G, 8-OH-G).

Specificity: 8-OHDG in all species. No observable crossreactivity for the following analogues: guanosine (G), 7methylG, 6SHG, 8bromoG, dA, dC, dT, dI, dU, dG, O6methyldG, 8OHdA, guanine (Gua), O6methylGua, 8OHGua, uric acid, Urea, creatine, creatinine Slight but minimal (<1%) crossreactivity for the following analogues: 8sulfhydrylG and 8OHG 1

Application Details

Application Notes: Tested for IHC and ELISA. User should determine optimum titer for each application.

Purification: Prepared as ascites then ammonium sulphate purified

Buffer: Lyophilized as 100 ug/mL IgG in10mM Phosphate buffered saline, pH7.4 containing 1.0% BSA.

Storage: Less than 20°C.

Research Area: DNA/RNA

Restrictions: For Research Use only

Images

Epidermis from hairless mouse by chronic UVB irradiation after 4 weeks of treatment stained with N45.1. (Y.Hattori, et.al.: J Invest Dermatol 107, p733-737, 1997)

Publications

Hattori, Nishigori, Tanaka et al.: "8-hydroxy-2'-deoxyguanosine is increased in epidermal cells of hairless mice after chronic ultraviolet B exposure." in: The Journal of investigative dermatology, Vol. 107, Issue 5, pp. 733-7, 1996 (PubMed).

Toyokuni, Tanaka, Hattori et al.: "Quantitative immunohistochemical determination of 8-hydroxy-2'-deoxyguanosine by a monoclonal antibody N45.1: its application to ferric nitrilotriacetate-induced renal carcinogenesis model." in: Laboratory investigation; a journal of technical methods and pathology, Vol. 76, Issue 3, pp. 365-74, 1997 (PubMed).

Tanaka, Nishiyama, Okada et al.: "Induction and nuclear translocation of thioredoxin by oxidative damage in the mouse kidney: independence of tubular necrosis and sulfhydryl depletion." in: Laboratory investigation; a journal of technical methods and pathology, Vol. 77, Issue 2, pp. 145-55, 1997 (PubMed).

Ihara, Toyokuni, Uchida et al.: "Hyperglycemia causes oxidative stress in pancreatic beta-cells of GK rats, a model of type 2 diabetes." in: Diabetes, Vol. 48, Issue 4, pp. 927-32, 1999 (PubMed).

Ichiseki, Kaneuji, Katsuda et al.: "DNA oxidation injury in bone early after steroid administration is involved in the pathogenesis of steroid-induced osteonecrosis." in: Rheumatology (Oxford, England), Vol. 44, Issue 4, pp. 456-60, 2005 (PubMed).

Lee, Cho, Yokozawa: "Protective effect of persimmon (Diospyros kaki) peel proanthocyanidin against oxidative damage under H2O2-induced cellular senescence." in: Biological & pharmaceutical bulletin, Vol. 31, Issue 6, pp. 1265-9, 2008 (PubMed).

Sato, Harada, Ikeda et al.: "Hepatic stellate cells are activated around central scars of focal nodular hyperplasia of the liver--a potential mechanism of central scar formation." in: Human pathology, Vol. 40, Issue 2, pp. 181-8, 2009 (PubMed).

Ribaldo, Souza, Biswas et al.: "Green tea (Camellia sinensis) attenuates nephropathy by downregulating Nox4 NADPH oxidase in diabetic spontaneously hypertensive rats." in: The Journal of nutrition, Vol. 139, Issue 1, pp. 96-100, 2008 (PubMed).

Du, Wooten, Gearing et al.: "Age-associated oxidative damage to the p62 promoter: implications for Alzheimer disease." in: Free radical biology & medicine, Vol. 46, Issue 4, pp. 492-501, 2009 (PubMed).

Iqbal, Okazaki, Okada: "Curcumin attenuates oxidative damage in animals treated with a renal carcinogen, ferric nitrilotriacetate (Fe-NTA): implications for cancer prevention." in: Molecular and cellular biochemistry, Vol. 324, Issue 1-2, pp. 157-64, 2009 (PubMed).

Sakano, Takahashi, Wang et al.: "Plasma 3-nitrotyrosine, urinary 8-isoprostane and 8-OHdG among healthy Japanese people." in: Free radical research, Vol. 43, Issue 2, pp. 183-92, 2009 (PubMed).

Song, Li, Ootsuyama et al.: "Urea, the most abundant component in urine, cross-reacts with a commercial 8-OH-dG ELISA kit and contributes to overestimation of urinary 8-OH-dG." in: Free radical biology & medicine, Vol. 47, Issue 1, pp. 41-6, 2009 (PubMed).

Silva, Rosales, Biswas et al.: "Diabetic retinal neurodegeneration is associated with mitochondrial oxidative stress and is improved by an angiotensin receptor blocker in a model combining hypertension and diabetes." in: Diabetes, Vol. 58, Issue 6, pp. 1382-90, 2009 (PubMed).

Min, Park, Kim et al.: "Effect of oxidative stress on birth sizes: consideration of window from mid pregnancy to delivery." in: Placenta, Vol. 30, Issue 5, pp. 418-23, 2009 (PubMed).

Yamazaki, Miwa, Toyokuni et al.: "Effect of edaravone, a novel free radical scavenger, supplemented to cardioplegia on myocardial function after cardioplegic arrest: in vitro study of isolated rat heart." in: Heart and vessels, Vol. 24, Issue 3, pp. 228-35, 2009 (PubMed).

Herbas, Thekisoe, Inoue et al.: "The effect of alpha-tocopherol transfer protein gene disruption on Trypanosoma congolense infection in mice." in: Free radical biology & medicine, Vol. 47, Issue 10, pp. 1408-13, 2009 (PubMed).