8-Hydroxyguanosine (8-OHDG) antibody

Details for Product No. ABIN335419
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Antigen
Reactivity
Chemical
(30), (2), (1), (1), (1)
Host
Mouse
(16), (16)
Clonality (Clone)
Monoclonal ()
Application
Immunohistochemistry (IHC), ELISA
(22), (15), (9), (6), (5), (5), (2), (2), (2), (1)
Pubmed 21 references available
Quantity 100 μg
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Immunogen 8-OHdG-conjugated Keyhole Limpet Hemocyanin
Clone N45-1
Isotype IgG1 kappa
Specificity 8-OHDG in all species. No observable crossreactivity for the following analogues: guanosine (G), 7methylG, 6SHG, 8bromoG, dA, dC, dT, dI, dU, dG, O6methyldG, 8OHdA, guanine (Gua), O6methylGua, 8OHGua, uric acid, Urea, creatine, creatinine Slight but minimal (<1 % ) crossreactivity for the following analogues: 8sulfhydrylG and 8OHG 1
Purification Prepared as ascites then ammonium sulphate purified
Alternative Name 8 Hydroxyguanosine (8-OHDG)
Target Type Chemical
Background 8-hydroxy-2'-deoxyguanosine (8-OHdG) is a product of oxidatively damaged DNA formed by hydroxy radical, singlet oxygen and direct photodynamic action. Anti 8-OHdG monoclonal antibody (clone N45.1) is highly specific for 8-OHdG, and is suitable for immunohistochemistry. Cross reactivity have been checked for 8-OHdG analogues (guanosine(G),7-methyl-G, 6-SH-G, 8-Bromo-G, dA, dC, dT, dI, dU, dG, O6-methyl-dG, 8-OHdA, guanine(Gua),O6-methyl-Gua, 8-OH-Gua, uric acid, urea, creatine, creatinine, 8-sulfhydryl-G, 8-OH-G).
Research Area DNA/RNA
Application Notes Tested for IHC and ELISA. User should determine optimum titer for each application.
Restrictions For Research Use only
Format Lyophilized
Buffer Lyophilized as 100 ug/mL IgG in10 mM Phosphate buffered saline, pH7.4 containing 1.0 % BSA.
Storage -20 °C
Supplier Images
anti-8-Hydroxyguanosine (8-OHDG) antibody Epidermis from hairless mouse by chronic UVB irradiation after 4 weeks of treatment s...
Product cited in: Raz, Zhang, Zhou et al.: "Role of Rac1 GTPase in NADPH oxidase activation and cognitive impairment following cerebral ischemia in the rat." in: PLoS ONE, Vol. 5, Issue 9, pp. e12606, 2010 (PubMed).

Herbas, Ueta, Ichikawa et al.: "Alpha-tocopherol transfer protein disruption confers resistance to malarial infection in mice." in: Malaria journal, Vol. 9, pp. 101, 2010 (PubMed).

Freeman, Parvizi, Della Valle et al.: "Reactive oxygen and nitrogen species induce protein and DNA modifications driving arthrofibrosis following total knee arthroplasty." in: Fibrogenesis & tissue repair, Vol. 2, Issue 1, pp. 5, 2009 (PubMed).

Celino, Yamaguchi, Miura et al.: "Arsenic inhibits in vitro spermatogenesis and induces germ cell apoptosis in Japanese eel (Anguilla japonica)." in: Reproduction (Cambridge, England), Vol. 138, Issue 2, pp. 279-87, 2009 (PubMed).

Fujihara, Nagai, Sussan et al.: "Chronic cigarette smoke causes oxidative damage and apoptosis to retinal pigmented epithelial cells in mice." in: PLoS ONE, Vol. 3, Issue 9, pp. e3119, 2008 (PubMed).

Betsuyaku, Hamamura, Hata et al.: "Bronchiolar chemokine expression is different after single versus repeated cigarette smoke exposure." in: Respiratory research, Vol. 9, pp. 7, 2008 (PubMed).

Choi, Ryu, Kim et al.: "Modulation of the purinergic P2X7 receptor attenuates lipopolysaccharide-mediated microglial activation and neuronal damage in inflamed brain." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 27, Issue 18, pp. 4957-68, 2007 (PubMed).

Albrecht, Knaapen, Becker et al.: "The crucial role of particle surface reactivity in respirable quartz-induced reactive oxygen/nitrogen species formation and APE/Ref-1 induction in rat lung." in: Respiratory research, Vol. 6, pp. 129, 2005 (PubMed).

Tam, Gao, Leung et al.: "Androgenic regulation of oxidative stress in the rat prostate: involvement of NAD(P)H oxidases and antioxidant defense machinery during prostatic involution and regrowth." in: The American journal of pathology, Vol. 163, Issue 6, pp. 2513-22, 2003 (PubMed).

Kuwano, Nakashima, Inoshima et al.: "Oxidative stress in lung epithelial cells from patients with idiopathic interstitial pneumonias." in: The European respiratory journal, Vol. 21, Issue 2, pp. 232-40, 2003 (PubMed).

Tanito, Nishiyama, Tanaka et al.: "Change of redox status and modulation by thiol replenishment in retinal photooxidative damage." in: Investigative ophthalmology & visual science, Vol. 43, Issue 7, pp. 2392-400, 2002 (PubMed).

Liardet, Scaletta, Panizzon et al.: "Protection against pyrimidine dimers, p53, and 8-hydroxy-2'-deoxyguanosine expression in ultraviolet-irradiated human skin by sunscreens: difference between UVB + UVA and UVB alone sunscreens." in: The Journal of investigative dermatology, Vol. 117, Issue 6, pp. 1437-41, 2002 (PubMed).

Kato, Kobune, Nakamura et al.: "Normalization of elevated hepatic 8-hydroxy-2'-deoxyguanosine levels in chronic hepatitis C patients by phlebotomy and low iron diet." in: Cancer research, Vol. 61, Issue 24, pp. 8697-702, 2001 (PubMed).

Aoshiba, Tamaoki, Nagai: "Acute cigarette smoke exposure induces apoptosis of alveolar macrophages." in: American journal of physiology. Lung cellular and molecular physiology, Vol. 281, Issue 6, pp. L1392-401, 2001 (PubMed).

Gottschling, Maronpot, Hailey et al.: "The role of oxidative stress in indium phosphide-induced lung carcinogenesis in rats." in: Toxicological sciences : an official journal of the Society of Toxicology, Vol. 64, Issue 1, pp. 28-40, 2001 (PubMed).

Miyamoto, Kishimoto, Shioji et al.: "Difference in thioredoxin expression in viral myocarditis in inbred strains of mice." in: Japanese circulation journal, Vol. 65, Issue 6, pp. 561-4, 2001 (PubMed).

Yamagami, Yamamoto, Kume et al.: "Formation of 8-hydroxy-2'-deoxyguanosine and 4-hydroxy-2-nonenal-modified proteins in rat liver after ischemia-reperfusion: distinct localization of the two oxidatively modified products." in: Antioxidants & redox signaling, Vol. 2, Issue 1, pp. 127-36, 2001 (PubMed).

Ahmed, Ueda, Nikaido et al.: "High levels of 8-hydroxy-2'-deoxyguanosine appear in normal human epidermis after a single dose of ultraviolet radiation." in: The British journal of dermatology, Vol. 140, Issue 2, pp. 226-31, 2000 (PubMed).

Kawashima, Bando, Nakamura et al.: "Cytoprotective effects of nitroglycerin in ischemia-reperfusion-induced lung injury." in: American journal of respiratory and critical care medicine, Vol. 161, Issue 3 Pt 1, pp. 935-43, 2000 (PubMed).

Kondo, Toyokuni, Iwasa et al.: "Persistent oxidative stress in human colorectal carcinoma, but not in adenoma." in: Free radical biology & medicine, Vol. 27, Issue 3-4, pp. 401-10, 1999 (PubMed).

Koyama, Wada, Sakuraba et al.: "Accelerated loss of islet beta cells in sucrose-fed Goto-Kakizaki rats, a genetic model of non-insulin-dependent diabetes mellitus." in: The American journal of pathology, Vol. 153, Issue 2, pp. 537-45, 1998 (PubMed).

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Catalog No. ABIN335419
992.20 $
Plus shipping costs $45.00
Quantity
Price
100 μg
992.20 $

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