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8-Hydroxyguanosine (8-OHDG) antibody

Details for Product No. ABIN335419, Supplier: Log in to see
Antigen
Reactivity
Chemical
27
1
1
1
1
Host
Mouse
14
14
Clonality (Clone)
Monoclonal ()
Application
Immunohistochemistry (IHC), ELISA
20
13
6
5
4
4
2
2
2
1
Supplier
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Immunogen 8-OHdG-conjugated Keyhole Limpet Hemocyanin
Clone N45-1
Isotype IgG1 kappa
Specificity 8-OHDG in all species. No observable crossreactivity for the following analogues: guanosine (G), 7methylG, 6SHG, 8bromoG, dA, dC, dT, dI, dU, dG, O6methyldG, 8OHdA, guanine (Gua), O6methylGua, 8OHGua, uric acid, Urea, creatine, creatinine Slight but minimal (<1 %) crossreactivity for the following analogues: 8sulfhydrylG and 8OHG 1
Purification Prepared as ascites then ammonium sulphate purified
Alternative Name 8 Hydroxyguanosine (8-OHDG)
Target Type Chemical
Background 8-hydroxy-2'-deoxyguanosine (8-OHdG) is a product of oxidatively damaged DNA formed by hydroxy radical, singlet oxygen and direct photodynamic action. Anti 8-OHdG monoclonal antibody (clone N45.1) is highly specific for 8-OHdG, and is suitable for immunohistochemistry. Cross reactivity have been checked for 8-OHdG analogues (guanosine(G),7-methyl-G, 6-SH-G, 8-Bromo-G, dA, dC, dT, dI, dU, dG, O6-methyl-dG, 8-OHdA, guanine(Gua),O6-methyl-Gua, 8-OH-Gua, uric acid, urea, creatine, creatinine, 8-sulfhydryl-G, 8-OH-G).
Research Area DNA/RNA
Pathways
Application Notes Tested for IHC and ELISA. User should determine optimum titer for each application.
Restrictions For Research Use only
Format Lyophilized
Buffer Lyophilized as 100 ug/mL IgG in10 mM Phosphate buffered saline, pH7.4 containing 1.0 % BSA.
Storage -20 °C
Supplier Images
 image for anti-8-Hydroxyguanosine (8-OHDG) antibody (ABIN335419) Epidermis from hairless mouse by chronic UVB irradiation after 4 weeks of treatment s...
Product cited in: Raz, Zhang, Zhou et al.: "Role of Rac1 GTPase in NADPH oxidase activation and cognitive impairment following cerebral ischemia in the rat." in: PLoS ONE, Vol. 5, Issue 9, pp. e12606, 2010 (PubMed).

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Freeman, Parvizi, Della Valle et al.: "Reactive oxygen and nitrogen species induce protein and DNA modifications driving arthrofibrosis following total knee arthroplasty." in: Fibrogenesis & tissue repair, Vol. 2, Issue 1, pp. 5, 2009 (PubMed).

Celino, Yamaguchi, Miura et al.: "Arsenic inhibits in vitro spermatogenesis and induces germ cell apoptosis in Japanese eel (Anguilla japonica)." in: Reproduction (Cambridge, England), Vol. 138, Issue 2, pp. 279-87, 2009 (PubMed).

Fujihara, Nagai, Sussan et al.: "Chronic cigarette smoke causes oxidative damage and apoptosis to retinal pigmented epithelial cells in mice." in: PLoS ONE, Vol. 3, Issue 9, pp. e3119, 2008 (PubMed).

Betsuyaku, Hamamura, Hata et al.: "Bronchiolar chemokine expression is different after single versus repeated cigarette smoke exposure." in: Respiratory research, Vol. 9, pp. 7, 2008 (PubMed).

Choi, Ryu, Kim et al.: "Modulation of the purinergic P2X7 receptor attenuates lipopolysaccharide-mediated microglial activation and neuronal damage in inflamed brain." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 27, Issue 18, pp. 4957-68, 2007 (PubMed).

Albrecht, Knaapen, Becker et al.: "The crucial role of particle surface reactivity in respirable quartz-induced reactive oxygen/nitrogen species formation and APE/Ref-1 induction in rat lung." in: Respiratory research, Vol. 6, pp. 129, 2005 (PubMed).

Tam, Gao, Leung et al.: "Androgenic regulation of oxidative stress in the rat prostate: involvement of NAD(P)H oxidases and antioxidant defense machinery during prostatic involution and regrowth." in: The American journal of pathology, Vol. 163, Issue 6, pp. 2513-22, 2003 (PubMed).

Kuwano, Nakashima, Inoshima et al.: "Oxidative stress in lung epithelial cells from patients with idiopathic interstitial pneumonias." in: The European respiratory journal, Vol. 21, Issue 2, pp. 232-40, 2003 (PubMed).

Tanito, Nishiyama, Tanaka et al.: "Change of redox status and modulation by thiol replenishment in retinal photooxidative damage." in: Investigative ophthalmology & visual science, Vol. 43, Issue 7, pp. 2392-400, 2002 (PubMed).

Liardet, Scaletta, Panizzon et al.: "Protection against pyrimidine dimers, p53, and 8-hydroxy-2'-deoxyguanosine expression in ultraviolet-irradiated human skin by sunscreens: difference between UVB + UVA and UVB alone sunscreens." in: The Journal of investigative dermatology, Vol. 117, Issue 6, pp. 1437-41, 2002 (PubMed).

Kato, Kobune, Nakamura et al.: "Normalization of elevated hepatic 8-hydroxy-2'-deoxyguanosine levels in chronic hepatitis C patients by phlebotomy and low iron diet." in: Cancer research, Vol. 61, Issue 24, pp. 8697-702, 2001 (PubMed).

Aoshiba, Tamaoki, Nagai: "Acute cigarette smoke exposure induces apoptosis of alveolar macrophages." in: American journal of physiology. Lung cellular and molecular physiology, Vol. 281, Issue 6, pp. L1392-401, 2001 (PubMed).

Gottschling, Maronpot, Hailey et al.: "The role of oxidative stress in indium phosphide-induced lung carcinogenesis in rats." in: Toxicological sciences : an official journal of the Society of Toxicology, Vol. 64, Issue 1, pp. 28-40, 2001 (PubMed).

Miyamoto, Kishimoto, Shioji et al.: "Difference in thioredoxin expression in viral myocarditis in inbred strains of mice." in: Japanese circulation journal, Vol. 65, Issue 6, pp. 561-4, 2001 (PubMed).

Yamagami, Yamamoto, Kume et al.: "Formation of 8-hydroxy-2'-deoxyguanosine and 4-hydroxy-2-nonenal-modified proteins in rat liver after ischemia-reperfusion: distinct localization of the two oxidatively modified products." in: Antioxidants & redox signaling, Vol. 2, Issue 1, pp. 127-36, 2001 (PubMed).

Ahmed, Ueda, Nikaido et al.: "High levels of 8-hydroxy-2'-deoxyguanosine appear in normal human epidermis after a single dose of ultraviolet radiation." in: The British journal of dermatology, Vol. 140, Issue 2, pp. 226-31, 2000 (PubMed).

Kawashima, Bando, Nakamura et al.: "Cytoprotective effects of nitroglycerin in ischemia-reperfusion-induced lung injury." in: American journal of respiratory and critical care medicine, Vol. 161, Issue 3 Pt 1, pp. 935-43, 2000 (PubMed).

Kondo, Toyokuni, Iwasa et al.: "Persistent oxidative stress in human colorectal carcinoma, but not in adenoma." in: Free radical biology & medicine, Vol. 27, Issue 3-4, pp. 401-10, 1999 (PubMed).

Koyama, Wada, Sakuraba et al.: "Accelerated loss of islet beta cells in sucrose-fed Goto-Kakizaki rats, a genetic model of non-insulin-dependent diabetes mellitus." in: The American journal of pathology, Vol. 153, Issue 2, pp. 537-45, 1998 (PubMed).