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Donkey anti-Sheep (Ovine) IgG (H&L) Antibody (Alkaline Phosphatase)

Antigen

IgG
Epitope
Clonality Polyclonal
Host
Alternatives

Donkey
Reactivity
Alternatives

Sheep (Ovine)
Conjugate
Alternatives Alkaline Phosphatase (AP)
Application
Immunoassay (IA), ELISA, Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC)
Catalog no. ABIN336504
Quantity 1 mg  (Variants)
Price 121.43 $   Plus shipping costs $35.00
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Additional Information
Application Details Alternatives Variants back to top

Alternative name IgG (H&L)
Immunogen Donkey antiserum was obtained from animals of US origin and under the care of a registered veterinarian. Country of Origin: Donkey antiserum was obtained from animals of US origin and under the care of a registered veterinarian.
Specificity This antibody reacts with the heavy chains on sheep IgG and with the light chains on all sheep immunoglobulins based on IEP. Cross reactivity: No reactivity is observed to non-immunoglobulin sheep serum proteins based on IEP.

Application Details
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Application Notes This conjugate is suitable for all immunoassay applications. The optimal working dilution should be determined by the investigator. Suggested starting dilution(s): 1:500-1:2,000 for ELISA/Western blot 1:20. 1:2,000 for Immunohistochemistry 1:50. 1:5,000 for Immunocytochemistry
Purity Antibody was affinity purified using solid phase Sheep IgG
Purification Antibody was affinity purified using solid phase Sheep IgG
Buffer 30 mM Triethanolamine, pH 7.2, 5 mM Magnesium Chloride, 0.1 mM Zinc Chloride, 1 % (w/v) BSA, Protease/IgG free. Preservative: 0.05 % (w/v) Sodium Azide
Storage Store undiluted liquid at 2-8 °C. For storage at -20 °C dilute with an equal volume of glycerol to prevent loss of enzymatic activity. Prepare working dilution prior to use and then discard. Shelf Life:1 year from date of receipt
Restrictions For Research Use only

Alternatives
Application Details Variants back to top

Alternatives for antigen "IgG", type "Secondary Antibodies"
Hosts Goat (1061), Rabbit (611), Donkey (490), Chicken (186), Sheep (78), Mouse (65), Rat (9), Duck (6), Guinea Pig (6), Horse (4)
Reactivities Rabbit (517), Mouse (Murine) (451), Human (407), Goat (293), Rat (Rattus) (253), Guinea Pig (141), Sheep (Ovine) (111), Hamster (63), Cow (Bovine) (45), Chicken (41), Syrian Hamster (37), Cat (Feline) (36), Dog (Canine) (33), Llama (30), Donkey (25), Horse (Equine) (14), Monkey (13), Pig (Porcine) (5), Bird (Avian) (3), Armenian Hamster (1)
Conjugates HRP (304), FITC (244), Biotin (231), Alkaline Phosphatase (AP) (211), DyLight 488 (141), TRITC (136), Texas Red (TR) (108), DyLight 405 (87), DyLight 594 (87), DyLight 680 (87), DyLight 800 (87), Rhodamine (64), PE (19), Beads (18), 488-NHS Ester (13), DyLight 350 (13), DyLight 550 (13), DyLight 633 (13), DyLight 650 (13), RPE (12), DyLight 549 (6), Agarose Beads (5), Colloidal Gold (10nm) (5), Cy5 (5), Colloidal Gold (5nm) (4), Cy3 (4), APC (3), SPRD (3), beta-Gal (3), AMCA (2), Colloidal Gold (20nm) (2), Cy2 (2), Poly-HRP (2), Alexa Fluor 647 (1), Colloidal Gold (15nm) (1), FL (1), PE,RPE (1)
Epitopes H&L (1367), Fc specific (73), Fab (1)