Goat anti-Human IgG, Fc Antibody (HRP)

Antigen: IgG, Fc

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Conjugate: HRP

Application: Immunoassay (IA), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)

Catalog no.: ABIN336612

Additional Information

Alternative name: IgG Fc

Immunogen: Goat antiserum was obtained from animals of US origin and under the care of a registered veterinarian. Country of Origin: Goat antiserum was obtained from animals of US origin and under the care of a registered veterinarian.

Format: Lyophilized

Description: This secondary antibody exihibits minimal cross reactivity with human IgA + IgM

Specificity: This antibody reacts with the heavy chains on human IgG based on IEP. Cross reactivity: No reactivity is observed with the light chains on human immunoglobulins. No reactivity is observed to human IgM or IgA based on IEP.

Application Details

Application Notes: This conjugate is suitable for all immunoassay applications. The optimal working dilution should be determined by the investigator. Suggested starting dilution(s): 1:500-1:5,000 for Immunohistochemistry 1:10,000-1:50,000 for ELISA/Western blot

Purity: Affinity purified using solid phase Human IgG

Purification: Affinity purified using solid phase Human IgG

Buffer: 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. Preservative: 0.1 % (v/v) Kathon CG – Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase

Storage: Undiluted conjugate is stable for 6-8 weeks at 2-8 °C. For long term storage, dilute with 50% glycerol and store at -20 °C as a liquid. Prepare working dilution prior to use and then discard.

Restrictions: For Research Use only