Rabbit anti-Mouse IgG Antibody (HRP)

Catalog No. ABIN336836

Quick Overview for Rabbit anti-Mouse IgG Antibody (HRP) (ABIN336836)

Target: IgG

Clonality: Polyclonal

Conjugate: HRP

Application: ELISA, Immunohistochemistry (IHC), Western Blotting (WB), Immunoassay (IA)

Product Details

Reactivity: Mouse

Host: Rabbit

Purpose: Rabbit anti-Mouse IgG (H&L) - Affinity Pure, HRP Conjugate, min x w/human serum proteins

Specificity: Based on IEP, this antibody reacts with: • heavy (i³) chains on mouse IgG • light chains on all mouse immunoglobulins

Cross-Reactivity (Details): Based on IEP, no reactivity is observed to: • non-immunoglobulin mouse serum proteins • human serum proteins

Purification: Affinity purified using solid phase Mouse IgG

Purity: Affinity purified antibody is > 95 % based on SDS-PAGE. E 1 % at 280 nm = 13.0

Immunogen: Purified Mouse IgG, whole molecule

Application Details

Application Notes: This conjugate is suitable for all immunoassay applications.
The optimal working dilution should be determined by the investigator. Suggested starting dilution: 1:500-1:5,000 for Immunohistochemistry 1:10,000-1:50,000 for ELISA/Western blot

Comment:

Country of Origin: Rabbit antiserum was obtained from animals of US origin and under the care of a registered veterinarian.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Rehydrate with 1.1 mL of deionized water and let stand 30 minutes at room temperature to dissolve. (Product has been overfilled to ensure complete recovery.) Centrifuge to remove any particulates. Prepare fresh working dilution daily.

Concentration: 1.0 mg/mL

Buffer: PBS, 1 % BSA & 0.1 % proclin 150, contains 0.1 % (v/v) Proclin 150

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: 1 year from date of receipt. Prepare working dilution prior to use and then discard.

Storage: 4 °C

Storage Comment: Store freeze-dried powder at 2-8 °C.

Expiry Date: 12 months

Target Details

Target: IgG

Target Type: Antibody