Fibronectin antibody

Catalog No. ABIN336929

The Rabbit Polyclonal anti-Fibronectin antibody is suitable to detect Fibronectin in samples from Human. It has been validated for IHC and IHC (p).

Quick Overview for Fibronectin antibody (ABIN336929)

Target: Fibronectin

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Fibronectin antibody is un-conjugated

Application: Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-Fibronectin Antibody

Specificity: This antibody reacts with human fibronectin in liver, tonsil, skin and kidney. Molecular Weight: Non reduced 400-440 kDa, reduced 200-220 kDa.

Immunogen: Purified human Fibronectin from human plasma

Application Details

Application Notes: Immunohistochemistry (IHC) 1:100-1:400 for 30 minute at room temp. using polymer system or streptavidin-biotin system. Formalin-fixed paraffin-embedded (FFPE) tissue sections and cell smears require antigen retriever, boiling tissue in 10 mM citrate buffer, pH 6.0 for 10-20 minute, followed by cooling at room temp. for 20 minutes. The optimum dilution should be determined by the individual lab. Positive Control: Human kidney. Cellular Localization: Connective tissue matrix and basement membrane.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Immunized serum in10 mM PO4, 150 mM NaCl, pH 7.4, 0.05% sodium azide. HostRabbit ReagentConc. antibody

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Target Details for Fibronectin

Target: Fibronectin

Background: Fibronectin is a glycoprotein present in a soluble dimeric form in plasma, and in a dimeric or multimeric form at the cell surface and extracellular matrix. Fibronectin is involved in cell adhesion and migration process including embryogenesis, wound healing, blood coagulation, host defense, and metastasis. They also occur as dimmer of two 200 kDa subunits. Fibronectin has been implicated in carcinoma development in lung cancer. Fibronectin expression is increased especially in non-small cell lung carcinoma. The adhesion of lung carcinoma cells to Fibronectin enhances tumorgenecity and confers resistance to apoptosis induced by standard chemotherapeutic agents.