ACAP1 antibody (AA 728-740)

Catalog No. ABIN337010

The Goat Polyclonal anti-ACAP1 antibody is suitable to detect ACAP1 in samples from Human, Mouse, Rat, Cow, Dog, Monkey and Rabbit. It has been validated for ELISA, WB, IHC and IHC (p).

Quick Overview for ACAP1 antibody (AA 728-740) (ABIN337010)

Target: ACAP1 (ArfGAP with Coiled-Coil, Ankyrin Repeat and PH Domains 1 (ACAP1))

Reactivity: Human, Mouse, Rat, Cow, Dog, Monkey, Rabbit

Host: Goat

Clonality: Polyclonal

Conjugate: This ACAP1 antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-ACAP1 Antibody

Binding Specificity: AA 728-740

Specificity: Human ACAP1 / CENTB1.

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Dog, Bovine, Elephant, Panda, Rabbit, Opossum (100%) Horse, Pig (92%) Bat (85%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide C-EKLSRRSHDLHTL from the C-terminus of human ACAP1 / CENTB1 (NP_055531.1). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Dog, Bovine, Elephant, Panda, Rabbit, Opossum (100%), Horse, Pig (92%), Bat (85%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), Peptide-ELISA (1:32000), WB (1:32000, 0.5 - 2 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for ACAP1

Target: ACAP1 (ArfGAP with Coiled-Coil, Ankyrin Repeat and PH Domains 1 (ACAP1))

Alternative Name: CENTB1 / ACAP1

Background: Name/Gene ID: ACAP1

Synonyms: ACAP1, Centaurin, beta 1, Centaurin-beta-1, Cnt-b1, KIAA0050, CENTB1

Gene ID: 9744

NCBI Accession: NP_055531

UniProt: Q15027