ACAP1 antibody (C-Term)

Catalog No. ABIN185096

The Goat Polyclonal anti-ACAP1 antibody is suitable to detect ACAP1 in samples from Human. It has been validated for ELISA and WB.

Quick Overview for ACAP1 antibody (C-Term) (ABIN185096)

Target: ACAP1 (ArfGAP with Coiled-Coil, Ankyrin Repeat and PH Domains 1 (ACAP1))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This ACAP1 antibody is un-conjugated

Application: ELISA, Western Blotting (WB)

Grade: Verified

Product Details anti-ACAP1 Antibody

Binding Specificity: C-Term

Purpose: CENTB1 / ACAP1

Sequence: EKLSRRSHDL HTL

Cross-Reactivity: Cow, Dog, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-EKLSRRSHDLHTL, from the C Terminus of the protein sequence according to NP_055531.1.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 80 kDa band observed in Jurkat lysates (calculated MW of 81.5 kDa according to NP_055531.1). Recommended for use at 0.3-1 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:32000.

Comment:

Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2018) PMID: 30377371.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for ACAP1

Target: ACAP1 (ArfGAP with Coiled-Coil, Ankyrin Repeat and PH Domains 1 (ACAP1))

Alternative Name: ACAP1

Background: ACAP1, CENTB1, centaurin, beta 1, KIAA0050, KIAA0050 gene product, Arf GAP with coiled coil, ANK repeat and PH domains 1, ArfGAP with coiled-coil, ankyrin repeat and PH domains 1, centaurin, beta 1

Gene ID: 9744, 216859

NCBI Accession: NP_055531