EIF4ENIF1 antibody (AA 974-986)

Catalog No. ABIN337018

This Goat Polyclonal antibody specifically detects EIF4ENIF1 in WB, ELISA, IHC and IHC (p). It exhibits reactivity toward Human, Pig, Xenopus laevis, Dog, Horse, Rabbit and Bat.

Quick Overview for EIF4ENIF1 antibody (AA 974-986) (ABIN337018)

Target: EIF4ENIF1 (Eukaryotic Translation Initiation Factor 4E Nuclear Import Factor 1 (EIF4ENIF1))

Reactivity: Human, Pig, Xenopus laevis, Dog, Horse, Rabbit, Bat

Host: Goat

Clonality: Polyclonal

Conjugate: This EIF4ENIF1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-EIF4ENIF1 Antibody

Binding Specificity: AA 974-986

Specificity: Human EIF4ENIF1. This antibody is expected to recognise isoform 1 (NP_062817.2) and isoform b (NP_001157974.1). Reported variants represent identical protein (NP_062817.2, NP_001157973.1).

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Baboon, Monkey, Shrew, Bat, Dog, Elephant, Panda, Rabbit, Horse, Pig, Armadillo, Xenopus (100%) Marmoset, Mouse, Rat, Bovine, Hamster, Opossum, Turkey, Chicken, Platypus (92%) Medaka, Zebrafish, Stickleback (85%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide C-AKVISVDELEYRQ from the C-terminus of human EIF4ENIF1 (NP_062817.2, NP_001157974.1). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Baboon, Monkey, Shrew, Bat, Dog, Elephant, Panda, Rabbit, Horse, Pig, Armadillo, Xenopus (100%), Marmoset, Mouse, Rat, Bovine, Hamster, Opossum, Turkey, Chicken, Platypus (92%), Medaka, Zebrafish, Stickleback (85%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), Peptide-ELISA, WB (0.25 - 0.5 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for EIF4ENIF1

Target: EIF4ENIF1 (Eukaryotic Translation Initiation Factor 4E Nuclear Import Factor 1 (EIF4ENIF1))

Alternative Name: EIF4ENIF1 / 4E-T

Background: Name/Gene ID: EIF4ENIF1

Synonyms: EIF4ENIF1, 4E-T, EIF4E transporter, EIF4E-transporter, Clast4

Gene ID: 56478

NCBI Accession: NP_062817, NP_001157974