EIF4ENIF1 antibody (C-Term)

Catalog No. ABIN185154

The Goat Polyclonal anti-EIF4ENIF1 antibody (ABIN185154) specifically detects EIF4ENIF1 in WB, ELISA and IHC. The antibody is reactive with Human samples.

Quick Overview for EIF4ENIF1 antibody (C-Term) (ABIN185154)

Target: EIF4ENIF1 (Eukaryotic Translation Initiation Factor 4E Nuclear Import Factor 1 (EIF4ENIF1))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This EIF4ENIF1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Grade: Verified

Product Details anti-EIF4ENIF1 Antibody

Binding Specificity: C-Term

Purpose: 4E-T / EIF4ENIF1

Sequence: AKVISVDELE YRQ

Specificity: This antibody is expected to recognise isoform 1 (NP_062817.2) and isoform b (NP_001157974.1). Reported variants represent identical protein (NP_062817.2, NP_001157973.1).

Cross-Reactivity: Cow, Dog, Human, Mouse, Pig, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-AKVISVDELEYRQ, from the C Terminus of the protein sequence according to NP_062817.2, NP_001157974.1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: In paraffin embedded Human Pancreas shows strong staining of cytoplasm in acinar cells-. Recommended concentration, 5-10 μg/mL.

Western Blot: Approx 140-150 kDa band observed in 293 lysates (predicted size of approx. 108 kDa according to NP_062817.1, however our observation agrees with that of Dostie et al (see below) ). Recommended for use at 0.25-0.5 μg/mL

Peptide ELISA: antibody detection limit dilution 1:32000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for EIF4ENIF1

Target: EIF4ENIF1 (Eukaryotic Translation Initiation Factor 4E Nuclear Import Factor 1 (EIF4ENIF1))

Alternative Name: EIF4ENIF1

Background: EIF4ENIF1, 4E-T, Clast4, FLJ21601, 2610509L04Rik, eukaryotic translation initiation factor 4E nuclear import factor 1, eIF4E-transporter, FLJ26551, OTTHUMP00000063276

Gene ID: 56478, 74203

NCBI Accession: NP_062817, NP_001157974