DKC1 antibody (AA 446-460)

Catalog No. ABIN337027

This Goat Polyclonal antibody specifically detects DKC1 in IHC, ELISA and IHC (p). It exhibits reactivity toward Human and Monkey.

Quick Overview for DKC1 antibody (AA 446-460) (ABIN337027)

Target: DKC1 (Dyskeratosis Congenita 1, Dyskerin (DKC1))

Reactivity: Human, Monkey

Host: Goat

Clonality: Polyclonal

Conjugate: This DKC1 antibody is un-conjugated

Application: Immunohistochemistry (IHC), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-DKC1 Antibody

Binding Specificity: AA 446-460

Specificity: Human DKC1.

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gorilla, Baboon, Monkey, Galago, Marmoset (100%) Gibbon, Hamster (93%) Mouse (87%) Horse, Pig, Armadillo (80%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide C-KRKRESESESDETPP from an internal region of human DKC1 (NP_001354.1). Percent identity by BLAST analysis: Human, Gorilla, Baboon, Monkey, Galago, Marmoset (100%), Gibbon, Hamster (93%), Mouse (87%), Horse, Pig, Armadillo (80%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), Peptide-ELISA (1:128000)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for DKC1

Target: DKC1 (Dyskeratosis Congenita 1, Dyskerin (DKC1))

Alternative Name: DKC1 / Dyskerin

Background: Name/Gene ID: DKC1

Synonyms: DKC1, CBF5, Cbf5p homolog, Dyskerin, DKC, NAP57, NOLA4, Nucleolar protein NAP57, XAP101, CBF5 homolog, DKCX

Gene ID: 1736

NCBI Accession: NP_001354

UniProt: O60832

Pathways: Telomere Maintenance