CCKBR antibody (AA 251-266)

Catalog No. ABIN337053

The Goat Polyclonal anti-CCKBR antibody (ABIN337053) specifically detects CCKBR in WB, ELISA, IHC and IHC (p). The antibody is reactive with Human samples.

Quick Overview for CCKBR antibody (AA 251-266) (ABIN337053)

Target: CCKBR (Cholecystokinin B Receptor (CCKBR))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This CCKBR antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-CCKBR Antibody

Binding Specificity: AA 251-266

Specificity: Human CCKBR.

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon (100%) Elephant (94%) Monkey, Hamster (88%) Marmoset, Mouse, Rabbit (81%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide C-FDGDSDSDSQSRVRNQ from an internal region of human CCKBR (NP_795344.1). Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon (100%), Elephant (94%), Monkey, Hamster (88%), Marmoset, Mouse, Rabbit (81%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), Peptide-ELISA (1:32000), WB (0.1 - 0.3 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for CCKBR

Target: CCKBR (Cholecystokinin B Receptor (CCKBR))

Alternative Name: CCKBR / Cckb

Background: Name/Gene ID: CCKBR
Subfamily: Cholecystokinin
Family: GPCR

Synonyms: CCKBR, CCKB, Cholecystokinin-2 receptor, CCK-BR, CCK2 receptor, CCK2-R, CCKB-R, Cholecystokinin-B, GASR, CCK-B, CCK-B receptor, CCK2R, Cckb receptor, CCKRB, Cholecystokinin B receptor, Gastrin receptor

Gene ID: 887

NCBI Accession: NP_795344

UniProt: P32239

Pathways: Positive Regulation of Peptide Hormone Secretion, Feeding Behaviour