ENPP1 antibody (AA 915-925)

Catalog No. ABIN337063

This Goat Polyclonal antibody specifically detects ENPP1 in IHC, ELISA and IHC (p). It exhibits reactivity toward Human and Rabbit.

Quick Overview for ENPP1 antibody (AA 915-925) (ABIN337063)

Target: ENPP1 (Ectonucleotide Pyrophosphatase/phosphodiesterase 1 (ENPP1))

Reactivity: Human, Rabbit

Host: Goat

Clonality: Polyclonal

Conjugate: This ENPP1 antibody is un-conjugated

Application: Immunohistochemistry (IHC), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-ENPP1 Antibody

Binding Specificity: AA 915-925

Specificity: Human ENPP1.

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Baboon, Monkey, Rabbit, Armadillo (100%) Marmoset, Mouse, Rat, Bovine, Hamster, Elephant, Panda (91%) Shrew, Bat, Horse, Opossum, Pufferfish (82%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide C-KTHLPTFSQED from the C-terminus of human ENPP1 (NP_006199.2). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Baboon, Monkey, Rabbit, Armadillo (100%), Marmoset, Mouse, Rat, Bovine, Hamster, Elephant, Panda (91%), Shrew, Bat, Horse, Opossum, Pufferfish (82%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (2.5 - 5 μg/mL), Peptide-ELISA (1:32000)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 2.5-5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for ENPP1

Target: ENPP1 (Ectonucleotide Pyrophosphatase/phosphodiesterase 1 (ENPP1))

Alternative Name: ENPP1 / PC1

Background: Name/Gene ID: ENPP1
Family: Phosphodiesterase - Other

Synonyms: ENPP1, Alkaline phosphodiesterase I, ARHR2, E-NPP 1, Ly-41 antigen, NPP1, NPPS, PC1, PDNP1, Alkaline phosphodiesterase 1, M6S1, PCA1, Pc-1

Gene ID: 5167

NCBI Accession: NP_006199

UniProt: P22413

Pathways: Regulation of Carbohydrate Metabolic Process