SNAP25 antibody (AA 193-206)

Catalog No. ABIN337119

The Goat Polyclonal anti-SNAP25 antibody (ABIN337119) specifically detects SNAP25 in WB, ELISA, IHC and IHC (p). The antibody is reactive with Human, Rat, Mouse, Dog, Cow, Chicken, Zebrafish (Danio rerio), Monkey, Hamster, Horse, Rabbit and Bat samples.

Quick Overview for SNAP25 antibody (AA 193-206) (ABIN337119)

Target: SNAP25 (Synaptosomal-Associated Protein, 25kDa (SNAP25))

Reactivity: Human, Rat, Mouse, Dog, Cow, Chicken, Zebrafish (Danio rerio), Monkey, Hamster, Horse, Rabbit, Bat

Host: Goat

Clonality: Polyclonal

Conjugate: This SNAP25 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-SNAP25 Antibody

Binding Specificity: AA 193-206

Specificity: Human SNAP25. This antibody is expected to recognise both reported isoforms NP_003072 and NP_570824.

Predicted Reactivity: Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Dog, Bat, Bovine, Horse, Rabbit, Opossum, Chicken, Platypus, Lizard, Newt, Salmon, Smelt, Stickleback, Pufferfish, Zebrafish (100%) Frog (93%) Xenopus (86%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide C-DEANQRATKMLGSG from the C-terminus of human SNAP25 (NP_003072.2, NP_570824.1). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Dog, Bat, Bovine, Horse, Rabbit, Opossum, Chicken, Platypus, Lizard, Newt, Salmon, Smelt, Stickleback, Pufferfish, Zebrafish (100%), Frog (93%), Xenopus (86%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (2.5 μg/mL), Peptide-ELISA (1:32000), WB (0.01 - 0.03 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 2.5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for SNAP25

Target: SNAP25 (Synaptosomal-Associated Protein, 25kDa (SNAP25))

Alternative Name: SNAP25

Background: Name/Gene ID: SNAP25
Family: SNAP-25

Synonyms: SNAP25, BA416N4.2, DJ1068F16.2, SNAP, SUP, Super protein, RIC4, SEC9, SNAP-25, RIC-4

Gene ID: 6616

NCBI Accession: NP_003072, NP_570824

UniProt: P60880

Pathways: Positive Regulation of Peptide Hormone Secretion, Hormone Transport, Synaptic Vesicle Exocytosis, Dicarboxylic Acid Transport