CBX1 antibody (AA 69-81)

Catalog No. ABIN337122

The Goat Polyclonal anti-CBX1 antibody (ABIN337122) specifically detects CBX1 in WB, ELISA, IHC and IHC (p). The antibody is reactive with Human, Mouse, Dog, Pig, Cow, Horse, Monkey, Bat and Hamster samples.

Quick Overview for CBX1 antibody (AA 69-81) (ABIN337122)

Target: CBX1 (Chromobox Homolog 1 (CBX1))

Reactivity: Human, Mouse, Dog, Pig, Cow, Horse, Monkey, Bat, Hamster

Host: Goat

Clonality: Polyclonal

Conjugate: This CBX1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-CBX1 Antibody

Binding Specificity: AA 69-81

Specificity: Human CBX1. Variants (NP_006798.1, NP_001120700.1) encode the same protein.

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Hamster, Elephant, Panda, Dog, Bat, Bovine, Horse, Pig (100%) Rat, Opossum (92%) Platypus, Lizard (85%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide C-QSQKTAHETDKSE from an internal region of human CBX1 (NP_006798.1, NP_001120700.1). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Hamster, Elephant, Panda, Dog, Bat, Bovine, Horse, Pig (100%), Rat, Opossum (92%), Platypus, Lizard (85%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (2.5 μg/mL), Peptide-ELISA (1:32000), WB (1:32000, 0.3 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 2.5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for CBX1

Target: CBX1 (Chromobox Homolog 1 (CBX1))

Alternative Name: CBX1 / HP1 Beta

Background: Name/Gene ID: CBX1

Synonyms: CBX1, CBX, Chromobox protein homolog 1, HP1 beta, HP1Hs-beta, Heterochromatin protein 1-beta, HP1Hsbeta, M31, Modifier 1 protein, MOD1, HP1 beta homolog, HP1-BETA, Chromobox homolog 1, Heterochromatin protein p25, p25beta

Gene ID: 10951

NCBI Accession: NP_006798, NP_001120700

UniProt: P83916