KIF5B antibody (AA 951-963)

Catalog No. ABIN337215

The Goat Polyclonal anti-KIF5B antibody (ABIN337215) specifically detects KIF5B in WB, IHC, ELISA and IHC (p). The antibody is reactive with Human, Dog, Cow, Pig, Rabbit and Monkey samples.

Quick Overview for KIF5B antibody (AA 951-963) (ABIN337215)

Target: KIF5B (Kinesin Family Member 5B (KIF5B))

Reactivity: Human, Dog, Cow, Pig, Rabbit, Monkey

Host: Goat

Clonality: Polyclonal

Conjugate: This KIF5B antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-KIF5B Antibody

Binding Specificity: AA 951-963

Specificity: Human KIF5B.

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Dog, Bovine, Rabbit, Pig (100%) Elephant, Panda, Horse (92%) Turkey, Platypus (85%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide C-QPVAVRGGGGKQV from the C-terminus of human KIF5B (NP_004512.1). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Dog, Bovine, Rabbit, Pig (100%), Elephant, Panda, Horse (92%), Turkey, Platypus (85%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (2.5 μg/mL), Peptide-ELISA (1:32000), WB (0.2 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 2.5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for KIF5B

Target: KIF5B (Kinesin Family Member 5B (KIF5B))

Alternative Name: Kinesin / KIF5B

Background: Name/Gene ID: KIF5B

Synonyms: KIF5B, Kinesin 1 (110-120kD), KINH, KNS, Kinesin family member 5B, Kinesin heavy chain, KNS1, UKHC, Kinesin-1 heavy chain, Ubiquitous kinesin heavy chain

Gene ID: 3799

NCBI Accession: NP_004512

UniProt: P33176

Pathways: Peptide Hormone Metabolism, Ribonucleoprotein Complex Subunit Organization