FHL1 antibody (AA 261-272)

Catalog No. ABIN337221

The Goat Polyclonal anti-FHL1 antibody (ABIN337221) specifically detects FHL1 in WB, ELISA, IHC and IHC (p). The antibody is reactive with Human, Mouse, Rat and Hamster samples.

Quick Overview for FHL1 antibody (AA 261-272) (ABIN337221)

Target: FHL1 (Four and A Half LIM Domains 1 (FHL1))

Reactivity: Human, Mouse, Rat, Hamster

Host: Goat

Clonality: Polyclonal

Conjugate: This FHL1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-FHL1 Antibody

Binding Specificity: AA 261-272

Specificity: Human FHL1. This antibody is expected to recognise isoforms 2( NP_001440.1) and 5 (NP_001153171.1). Since June 2009 the second N residue in the immunizing peptide is no longer representative as it has been substituted by Q at position 284 of NP_001440.2. Variants NP_001440.2, NP_001153176.1 and NP_001153172.1 represent identical protein.

Predicted Reactivity: Percent identity by BLAST analysis: Mouse, Rat, Hamster (100%) Human, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Sheep, Elephant, Bovine, Dog, Horse, Rabbit, Pig, Xenopus (92%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide CNKRFVFHNEQVY from an internal region of human FHL1 (NP_001440.1, NP_001153171.1). Percent identity by BLAST analysis: Mouse, Rat, Hamster (100%), Human, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Sheep, Elephant, Bovine, Dog, Horse, Rabbit, Pig, Xenopus (92%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (2.5 μg/mL), Peptide-ELISA (1:32000), WB (1:32000, 0.05 - 0.3 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 2.5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for FHL1

Target: FHL1 (Four and A Half LIM Domains 1 (FHL1))

Alternative Name: SLIM / FHL1

Background: Name/Gene ID: FHL1

Synonyms: FHL1, BA535K18.1, FHL1B, FHL1A, FLH1A, Four and a half LIM domains 1, FHL-1, KYOT, Skeletal muscle LIM-protein 1, SLIM-1, Skeletal muscle LIM-protein1, SLIMMER, XMPMA, KYO-T, LIM protein SLIMMER, SLIM, SLIM1

Gene ID: 2273

NCBI Accession: NP_001440, NP_001153171

UniProt: Q13642