EZH2 (Internal Region) antibody

Antigen: EZH2 (Internal Region)

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Catalog no.: ABIN337251

Additional Information

Swiss-Prot: Q15910

Immunogen: Synthetic peptide derived from the internal region of the human EZH2 (ENX1) protein.

Isotype: IgG  (Matching secondary antibodies)

Description: Gene: EZH2, Standard Gene Symbol: EZH2, Synonyms: EZH2, Enhancer of zeste, ENX-1, ENX1, EZH1, KMT6, KMT6A, MGC9169

Specificity: Recognizes the human EZH2 protein. On Western blots, it identifies bands at ~100kD and ~125kD, which may represent different isoforms of the protein. Reactivity has been confirmed with human Jurkat T cell leukemia, PC-3 prostate adenocarcinoma, BC-1 B cell lymphoma, Nalm-6 pre-B lymphocyte and HeLa cervical adenocarcinoma cell lysates by Western Blot and with formalin-fixed, paraffin-embedded (FFPE) human breast cancer, lymphoma, and normal prostate, esophagus, pancreas, stomach, liver, and brain tissues by immunohistochemistry. Species sequence homology: Mouse.

Application Details

Application Notes: ELISA (0.1 - 1 µg/ml), IHC-P (5 µg/ml), WB (0.5 - 2 µg/ml)

Concentration: 0.25 mg/ml

Purification: Immunoaffinity Chromatography

Buffer: PBS, pH 7.4, 0.1% sodium azide.

Storage: Long term: -20°C, Short term: +4°C, Avoid freeze-thaw cycles.

Restrictions: For Research Use only

Images

Human Testis (formalin-fixed, paraffin-embedded) stained with EZH2 antibody ABIN337251 at 5 ug/ml followed by biotinylated goat anti-rabbit IgG secondary antibody ABIN481713, alkaline phosphatase-streptavidin and chromogen.