EZH2 antibody (Internal Region)

Catalog No. ABIN337251

The Rabbit Polyclonal anti-EZH2 antibody (ABIN337251) specifically detects EZH2 in WB, IHC, ELISA and IHC (p). The antibody is reactive with Human samples.

Quick Overview for EZH2 antibody (Internal Region) (ABIN337251)

Target: EZH2 (Enhancer of Zeste Homolog 2 (EZH2))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EZH2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-EZH2 Antibody

Binding Specificity: Internal Region

Specificity: Recognizes the human EZH2 protein. On Western blots, it identifies bands at ~100kD and ~125kD, which may represent different isoforms of the protein. Reactivity has been confirmed with human Jurkat T cell leukemia, PC-3 prostate adenocarcinoma, BC-1 B cell lymphoma, Nalm-6 pre-B lymphocyte and HeLa cervical adenocarcinoma cell lysates by Western Blot and with formalin-fixed, paraffin-embedded (FFPE) human breast cancer, lymphoma, and normal prostate, esophagus, pancreas, stomach, liver, and brain tissues by immunohistochemistry. Species sequence homology: Mouse.

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide derived from the internal region of the human EZH2 (ENX1) protein.

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: ELISA (0.1 - 1 μg/mL), IHC, IHC-P (5 μg/mL), WB (0.5 - 2 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Western Blot: 0.5-2 μg/mL. Bands at ~100kD and ~125kD.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4, 0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for EZH2

Target: EZH2 (Enhancer of Zeste Homolog 2 (EZH2))

Alternative Name: EZH2

Background: Name/Gene ID: EZH2

Synonyms: EZH2, ENX1, Enhancer of zeste homolog 2, KMT6, KMT6A, Lysine N-methyltransferase 6, WVS2, Enhancer of zeste, ENX-1

Gene ID: 2146

UniProt: Q15910

Pathways: Retinoic Acid Receptor Signaling Pathway, Regulation of Muscle Cell Differentiation