TRIM5 antibody (AA 2-15)

Catalog No. ABIN337284

The Goat Polyclonal anti-TRIM5 antibody (ABIN337284) specifically detects TRIM5 in WB, ELISA, IHC and IHC (p). The antibody is reactive with Human and Monkey samples.

Quick Overview for TRIM5 antibody (AA 2-15) (ABIN337284)

Target: TRIM5 (Tripartite Motif Containing 5 (TRIM5))

Reactivity: Human, Monkey

Host: Goat

Clonality: Polyclonal

Conjugate: This TRIM5 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-TRIM5 Antibody

Binding Specificity: AA 2-15

Specificity: Human TRIM5. This antibody is expected to recognise all three human isoforms of this protein.

Predicted Reactivity: Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Baboon, Monkey (100%) Tamarin, Horse (93%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide ASGILVNVKEEVTC from the N-terminus of human TRIM5 (NP_149023.1, NP_149083.1, NP_149084.1). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Baboon, Monkey (100%), Tamarin, Horse (93%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), Peptide-ELISA (1:16000), WB (1 - 3 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for TRIM5

Target: TRIM5 (Tripartite Motif Containing 5 (TRIM5))

Alternative Name: TRIM5

Background: Name/Gene ID: TRIM5
Family: Tripartite Motif

Synonyms: TRIM5, RNF88, RING finger protein 88, Tripartite motif containing 5, TRIM5alpha, Tripartite motif protein TRIM5, Tripartite motif-containing 5

Gene ID: 85363

NCBI Accession: NP_149023, NP_149083, NP_149084

Pathways: Activation of Innate immune Response