SMUG1 antibody (AA 2-14)

Catalog No. ABIN337309

This Goat Polyclonal antibody specifically detects SMUG1 in WB, ELISA, IHC and IHC (p). It exhibits reactivity toward Human and Monkey.

Quick Overview for SMUG1 antibody (AA 2-14) (ABIN337309)

Target: SMUG1 (Single-Strand-Selective Monofunctional Uracil-DNA Glycosylase 1 (SMUG1))

Reactivity: Human, Monkey

Host: Goat

Clonality: Polyclonal

Conjugate: This SMUG1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-SMUG1 Antibody

Binding Specificity: AA 2-14

Specificity: Human SMUG1.

Predicted Reactivity: Percent identity by BLAST analysis: Human, Monkey (100%) Gorilla, Gibbon (92%) Horse (85%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide PQAFLLGSIHEPA-C from the N-terminus of human SMUG1 (NP_055126.1). Percent identity by BLAST analysis: Human, Monkey (100%), Gorilla, Gibbon (92%), Horse (85%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (2.5 μg/mL), Peptide-ELISA (1:32000), WB (0.5 - 2 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 2.5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for SMUG1

Target: SMUG1 (Single-Strand-Selective Monofunctional Uracil-DNA Glycosylase 1 (SMUG1))

Alternative Name: SMUG1

Background: Name/Gene ID: SMUG1

Synonyms: SMUG1, HMUDG, FDG, UNG3

Gene ID: 23583

NCBI Accession: NP_055126

UniProt: Q53HV7

Pathways: DNA Damage Repair