HYAL1 antibody (AA 325-339)

Catalog No. ABIN337333

This Goat Polyclonal antibody specifically detects HYAL1 in IHC, ELISA and IHC (p). It exhibits reactivity toward Human, Dog and Monkey.

Quick Overview for HYAL1 antibody (AA 325-339) (ABIN337333)

Target: HYAL1 (Hyaluronidase-1 (HYAL1))

Reactivity: Human, Dog, Monkey

Host: Goat

Clonality: Polyclonal

Conjugate: This HYAL1 antibody is un-conjugated

Application: Immunohistochemistry (IHC), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-HYAL1 Antibody

Binding Specificity: AA 325-339

Specificity: Human HYAL1. This antibody is expected to recognise isoform 1 (NP_009296.1), isoform 2 (NP_695014.1), isoform 3 (NP_695015.1) and isoform 5 (NP_695017.1). Reported variants represent identical protein (NP_009296.1, NP_149349.2, NP_695013.1).

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Dog (100%) Elephant, Panda, Bat, Horse, Pig (93%) Rat, Bovine, Rabbit (80%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide ENTRTKESCQAIKEY from an internal region of human HYAL1 (NP_009296.1, NP_695014.1, NP_695015.1, NP_695017.1,). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Dog (100%), Elephant, Panda, Bat, Horse, Pig (93%), Rat, Bovine, Rabbit (80%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), Peptide-ELISA (1:128000)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for HYAL1

Target: HYAL1 (Hyaluronidase-1 (HYAL1))

Alternative Name: HYAL1

Background: Name/Gene ID: HYAL1

Synonyms: HYAL1, FUS2, Hyaluronidase-1, Hyaluronoglucosaminidase 1, LUCA1, Hyaluronoglucosaminidase-1, Plasma hyaluronidase, LuCa-1, Tumor suppressor LUCA-1, HYAL-1, Lung carcinoma protein 1

Gene ID: 3373

NCBI Accession: NP_009296, NP_695014, NP_695015, NP_695017

UniProt: Q12794

Pathways: Glycosaminoglycan Metabolic Process