14-3-3 Binding Motif (pSer) antibody

Antigen: 14-3-3 Binding Motif

Epitope: pSer

Clonality: Polyclonal

Host: Rabbit

Application: Western Blotting (WB), Immunoprecipitation (IP), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Catalog no.: ABIN337475

Additional Information

Alternative name: 14-3-3 Binding Motif, pSer

Immunogen: Synthetic peptide, Synthetic phospho- (Ser) 14-3-3-binding-motif peptides.

Isotype: IgG  (Matching secondary antibodies)

Description: Gene: 14-3-3 Binding Motif

Specificity: Binds peptides and proteins containing a motif composed of phospho-Ser with proline at the +2 position and arginine or lysine at the -3 position. Antibody binding is phospho-specific and largely independent of other surrounding amino acids. Weakly cross-reacts with sequences containing phospho-Thr instead of phospho-Ser in this motif, with sequences containing phospho-Ser surrounded by Phe at the +1 position, with Arg/Lys at the -3 position. No cross-reactivity is observed with corresponding non-phosphorylated sequences or with other phospho- Thr/Ser/Tyr -containing motifs.

Application Details

Application Notes: WB (1:1000), IP (1:50), ELISA (1:1000), IHC-P

Purification: Immunoaffinity Chromatography

Buffer: 10 mM sodium HEPES, pH 7.5, 150 mM sodium chloride, 0.1 mg/ml BSA, 50% glycerol.

Restrictions: For Research Use only