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Goat anti-Rat IgG (Fc Region) Antibody (Alkaline Phosphatase (AP))

Name: Goat anti-Rat IgG (Fc Region) Antibody (Alkaline Phosphatase (AP))
SKU: ABIN339133
Availability: InStock

ELISA, IHC, WB Polyclonal IgG Alkaline Phosphatase (AP)

Catalog No. ABIN339133

$1,093.40

Plus shipping costs $50.00

500 μg

Shipping to: United States

Delivery in 11 to 15 Business Days

Quick Overview for Goat anti-Rat IgG (Fc Region) Antibody (Alkaline Phosphatase (AP)) (ABIN339133)

Target

IgG

Clonality

Polyclonal

Conjugate

Alkaline Phosphatase (AP)

Application

2571
2526
2306
1859
1470
918
917
402
391
232
232
100
91
82
68
34
29
24
18
17
16
12
12
11
9
9
8
7
7
6
4
2
2
1
1
1
1
1
1
1
1
1
1

ELISA, Immunohistochemistry (IHC), Western Blotting (WB)

Isotype

IgG

Binding Specificity
2936

374

350

148

131

92

65

29

29

28

28

5

5

2

1

1

1

1

1

1

1

1

1

1

1
Fc Region
Reactivity
804

754

684

453

451

244

211

209

194

154

123

118

99

97

81

76

70

58

33

27

27

27

27

26

13

7

6

5

5

4

4

4

3

3

3

3

3

3

3

2

2

2

1

1

1

1
Rat
Host
1854

1738

628

417

153

99

30

26

25

20

19

12

11
Goat

Specificity

Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase, anti-Goat Serum, Rat IgG, Rat IgG Fc and Rat Serum. No reaction was observed against anti-Pepsin, anti-Goat IgG F(c), Rat F (ab')2 or Bovine, Horse, and Human Serum Proteins.

Purification

Immunoaffinity purified

Immunogen

Rat IgG F(c) fragment. Label: Alkaline Phosphatase (Calf Intestine) (MW: 140000 daltons).

Type of Immunogen: Purified protein
Goat anti-Rat IgG (Heavy & Light Chain) antibody (Atto 488) - Preadsorbed
WB, FLISA, FM, DB Polyclonal IgG Atto 488

Rabbit anti-Mouse IgG antibody (HRP) - Preadsorbed
ELISA, WB, IHC (fro), IHC (p), IHC (rs) Polyclonal IgG HRP F(ab')2 fragment

Goat anti-Rat IgG antibody (HRP)
ELISA, WB, ICC, IHC (fro) Polyclonal IgG HRP

Goat anti-Rat IgG (Heavy & Light Chain) antibody (HRP) - Preadsorbed
ELISA, IHC, WB Polyclonal IgG HRP

Goat anti-Rat IgG antibody (Cy3) - Preadsorbed
FACS, WB, FLISA, FM, DB Polyclonal IgG Cy3

Goat anti-Rat IgG antibody (DyLight 488)
WB, FLISA, FM Polyclonal IgG DyLight 488

Goat anti-Rat IgG (Heavy & Light Chain) antibody (Atto 550) - Preadsorbed
WB, FLISA, FM Polyclonal IgG Atto 550

Goat anti-Rat IgG (Heavy & Light Chain) antibody (Atto 647N) - Preadsorbed
WB, FLISA, FM, DB Polyclonal IgG Atto 647N

Mouse anti-Rat IgG (Heavy & Light Chain) antibody (HRP)
ELISA, WB Polyclonal Mixture HRP

Goat anti-Rat IgG (Whole Molecule) antibody
ELISA, IHC, WB unconjugated F(ab')2 fragment

Application Notes

Approved: ELISA (1:1000), IHC, WB

Usage: Suitable for Western Blot, ELISA and Immunohistochemistry. ELISA: 1:1000 to 1:5000 using pNPP p-nitrophenyl phosphate as a substrate for 30 minutes at room temperature. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Comment

Target Species of Antibody: Rat

Restrictions

For Research Use only
Format

Liquid

Concentration

Lot specific

Buffer

0.05 M Tris -HCl, 0.15 M sodium chloride, 0.001 M magnesium chloride, 0.0001 M zinc chloride,  pH 8.0, 10 mg/mL BSA (IgG, protease free), 0.01 % sodium azide, 50 % glycerol.

Preservative

Sodium azide

Precaution of Use

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice

Do not freeze! Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
Do not add Sodium azide.
Dilute only prior to immediate use
Each reagent is stable for the period shown on the bottle label if stored as directed.

Storage

4 °C

Storage Comment

Store at 4°C. Do not freeze.
Target

IgG

Target Type

Antibody

Molecular Weight

140 kDa