Goat anti-Rat IgG (F(ab')2 Region) Antibody (HRP)

Catalog No. ABIN339163

Quick Overview for Goat anti-Rat IgG (F(ab')2 Region) Antibody (HRP) (ABIN339163)

Target: IgG

Clonality: Polyclonal

Conjugate: HRP

Application: ELISA, Immunohistochemistry (IHC), Western Blotting (WB)

Isotype: IgG

Product Details

Binding Specificity: F(ab')2 Region

Reactivity: Rat

Host: Goat

Specificity: Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, Rat IgG, Rat IgG F (ab')2 and Rat Serum. No reaction was observed against anti-Pepsin, anti-Goat IgG F(c), Rat IgG F(c) or Bovine, Horse and Human Serum Proteins.

Purification: Immunoaffinity purified

Immunogen: Rat IgG F (ab')2 fragment. Label: Horseradish Peroxidase (HRP).

Type of Immunogen: Purified protein

Application Details

Application Notes: Approved: ELISA (1:5000), IHC, WB

Usage: Suitable for Western Blot, ELISA, Immunohistochemistry and Immunoperoxidase electron microscopy. ELISA: 1:5000 to 1:20000 using ABTS as a substrate for 30 minutes at room temperature. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Comment:

Target Species of Antibody: Rat

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Sterile ddH2O

Concentration: Lot specific

Buffer: Lyophilized, PBS,  pH 7.6, 15 mg/mL BSA (IgG and Protease free).

Handling Advice: Avoid repeated freezing and thawing. Dilute only prior to immediate use
Centrifuge product if not completely clear after standing at room temperature.
Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details

Target: IgG

Target Type: Antibody