Rabbit anti-Sheep IgG (F(ab')2 Region) Antibody (HRP)

Catalog No. ABIN339206

Quick Overview for Rabbit anti-Sheep IgG (F(ab')2 Region) Antibody (HRP) (ABIN339206)

Target: IgG

Clonality: Polyclonal

Conjugate: HRP

Application: ELISA, Immunohistochemistry (IHC), Western Blotting (WB)

Isotype: IgG

Product Details

Binding Specificity: F(ab')2 Region

Reactivity: Sheep

Host: Rabbit

Specificity: Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum, Sheep IgG, Sheep IgG F (ab')2 and Sheep Serum. No reaction was observed against Sheep IgG F(c).

Purification: Immunoaffinity purified

Immunogen: Sheep IgG F (ab')2 fragment.

Type of Immunogen: Purified protein

Application Details

Application Notes: Approved: ELISA (1:1000 - 1:5000), IHC, WB

Usage: Suitable for use in Western Blot, Dot Blot, ELISA, Immunoperoxidase Electron Microscopy and Immunohistochemistry. ELISA: 1:1000-1:5000. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Comment:

Target Species of Antibody: Sheep

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4, 10 mg/mL BSA (IgG, protease free), 0.01 % Gentamicin sulfate, 40 % glycerol.

Preservative: Gentamicin sulfate

Precaution of Use: This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing. Dilute only prior to immediate use
Centrifuge product if not completely clear after standing at room temperature.
Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.

Storage: 4 °C,-20 °C

Storage Comment: Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.

Target Details

Target: IgG

Target Type: Antibody