Rabbit anti-Sheep IgG (F(ab')2 Region) Antibody (Biotin)

Catalog No. ABIN339233

Quick Overview for Rabbit anti-Sheep IgG (F(ab')2 Region) Antibody (Biotin) (ABIN339233)

Target: IgG

Clonality: Polyclonal

Conjugate: Biotin

Application: ELISA, Immunohistochemistry (IHC), Western Blotting (WB), Immunofluorescence (IF)

Product Details

Binding Specificity: F(ab')2 Region

Reactivity: Sheep

Host: Rabbit

Specificity: Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Rabbit Serum, Sheep IgG and Sheep Serum. No reaction was observed against Human Serum Proteins.

Purification: Immunoaffinity purified

Immunogen: Sheep IgG F (ab')2. Label: Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC).

Type of Immunogen: Purified protein

Application Details

Application Notes: Approved: ELISA (1:20000), IF, IHC, WB

Usage: Suitable for Western Blot, ELISA, Immunohistochemistry and Immunomicroscopy. ELISA: 1:20000 to 1:400000 using Peroxidase Conjugated Streptavidin and ABTS as a substrate for 30 minutes at room temperature. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Comment:

Target Species of Antibody: Sheep

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitution: Reconstitute, 1 mL sterile dH2O.

Concentration: Lot specific

Buffer: Lyophilized from PBS,  pH 7.2, 10 mg/mL BSA (IgG, protease free), 0.01 % sodium azide. Reconstitution: Reconstitute, 1 mL sterile dH2O.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Aliquot to Avoid repeated freezing and thawing.

Target Details

Target: IgG

Target Type: Antibody