Goat anti-Human IgM (Heavy Chain) Antibody (Alkaline Phosphatase (AP))

Catalog No. ABIN339442

Quick Overview for Goat anti-Human IgM (Heavy Chain) Antibody (Alkaline Phosphatase (AP)) (ABIN339442)

Target: IgM

Clonality: Polyclonal

Conjugate: Alkaline Phosphatase (AP)

Application: ELISA, Immunohistochemistry (IHC), Western Blotting (WB)

Isotype: IgG

Product Details

Binding Specificity: Heavy Chain

Reactivity: Human, Rat

Host: Goat

Specificity: Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Goat Serum, Rat IgM and Rat Serum. Specificity was confirmed by ELISA at less than 1 % cross-reactivity against other Rat or human heavy or light chain isotypes.

Purification: Immunoaffinity purified

Immunogen: Rat IgM heavy chain.

Type of Immunogen: Purified protein

Application Details

Application Notes: Approved: ELISA (1:500 - 1:2000), IHC, WB

Usage: Suitable for immunoblot, ELISA, immunohistochemistry and other phosphatase-antibody based enzymatic assays. ELISA: 1:500-1:2000 (Coat with1 μg of Rat IgM). The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Comment:

Target Species of Antibody: Rat

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: TBS, 1 mM magnesium chloride, 0.1 mM zinc chloride,  pH 8.0, 10 mg/mL BSA (IgG, protease free), 0.01 % sodium azide, 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze! Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
Do not add Sodium azide.
Dilute only prior to immediate use
Each reagent is stable for the period shown on the bottle label if stored as directed.

Storage: 4 °C

Storage Comment: Store at 4°C. Do not freeze.

Target Details

Target: IgM

Target Type: Antibody