Goat anti-Chicken IgM Antibody (Biotin)

Catalog No. ABIN339496

Quick Overview for Goat anti-Chicken IgM Antibody (Biotin) (ABIN339496)

Target: IgM

Clonality: Polyclonal

Conjugate: Biotin

Application: ELISA, Immunohistochemistry (IHC), Western Blotting (WB), Immunofluorescence (IF)

Product Details

Reactivity: Chicken

Host: Goat

Specificity: Assay by IEP resulted in a single precipitin arc against anti-biotin, anti-Goat Serum, Chicken IgM and Chicken Serum. No reaction was observed against Chicken IgG.

Purification: Immunoaffinity purified

Immunogen: Chicken IgM u chain.

Type of Immunogen: Purified protein

Application Details

Application Notes: Approved: ELISA (1:10000 - 1:40000), IF, IHC, WB

Usage: Suitable for use in Western Blot, ELISA, immunomicroscopy and immunohistochemistry. ELISA: 1:10000-1:40000, 1.0 μg of Chicken IgM was used in a standard capture ELISA with Streptavidin) and ABTS 1.46 mM/Liter for HRPO as a substrate for 30 minutes at RT. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Comment:

Target Species of Antibody: Chicken

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Sterile ddH2O

Concentration: Lot specific

Buffer: Lyophilized from PBS,  pH 7.2, 10 mg/mL BSA (IgG, protease free), 0.01 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid frequent freeze thaws. Store undiluted.

Storage: -20 °C,-80 °C

Storage Comment: Store at -20°C to -70°C. Stable for 1 year from date of receipt.

Target Details

Target: IgM

Target Type: Antibody