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|+1 404 474 4654|
|+1 888 205 9894 (TF)|
CD14 Molecule (CD14) antibody
|Clonality||Monoclonal (BiG 13)|
Alternatives Flow Cytometry (FACS), Western Blotting (WB), Functional Studies (Func), ELISA
|3 references available|
|Quantity||100ug (1 mg/ml)|
|Price||319.44 $ Plus shipping costs $45.00|
|Availability||Will be delivered in 4 to 5 Business Days|
|Immunogen||Monocytes of different species and immunoaffinity purified soluble human CD14|
|Description||BiG 13 represents an excellent marker for CD14. The CD14 glycoprotein, gp 55, is present on most monocytic and macrophages like cell types: monocytes, macrophages, Kupffer cells, pleural phagocytic cells and dendritic reticular cells. CD14 is also observed on granulocytes and activated or transformed B-cells. Furthermore CD14 is present in a soluble form in human serum, urine and other body fluids. The CD14 molecule has been reported to be a receptor for endotoxin.|
|Application Notes||Suitable for Immunostaining of CD14 positive cells (flow cytometry), ELISA, CD14 inhibition studies, Western Blot. 20ug/ml inhibits binding of 0.5 ug/ ml LPS to CD14, binding epitopes amino acid 9-13, 39-44, cross reacting with CD14 of cattle++, swine ++, dog+ horse+ Binding titre at human CD14 transfected CHO-cells (FACS): more than 100.000 Inhibits LPS induced IL1|
|Purification||Protein G purified|
|Buffer||0.1mg in PBS without NaN3|
|Research Area||Stem Cells, Innate Immunity, Hematopoietic Progenitors, Hematopoietic Stem Cells, CD Antigens, Surface Receptors of Immune Cells, Apoptosis/Necrosis, Immunology, Inflammation|
|Restrictions||For Research Use only|
Vidal, Labueta, Schiffrin et al.: "Soluble CD14 in human breast milk and its role in innate immune responses." in: Acta odontologica Scandinavica, Vol. 59, Issue 5, pp. 330-4, 2001 (PubMed).
Berner, Fuerll, Stelter et al.: "Elevated levels of lipopolysaccharide-binding protein and soluble CD14 in plasma in neonatal early-onset sepsis." in: Clinical and diagnostic laboratory immunology, Vol. 9, Issue 2, pp. 440-5, 2002 (PubMed).
Kato, Ogasawara, Homma et al.: "Lipopolysaccharide-binding protein critically regulates lipopolysaccharide-induced IFN-beta signaling pathway in human monocytes." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 172, Issue 10, pp. 6185-94, 2004 (PubMed).