CD14 Molecule (CD14) antibody

Antigen: CD14 Molecule (CD14)

Synonyms: CD14

Clonality: Monoclonal (BiG 13)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: Flow Cytometry (FACS), Western Blotting (WB), Functional Studies (Func), ELISA

Catalog no.: ABIN343168

Additional Information

Alternative name: CD14

Immunogen: Monocytes of different species and immunoaffinity purified soluble human CD14

Format: Lyophilized

Isotype: IgG1

Clone: BiG 13

Description: BiG 13 represents an excellent marker for CD14. The CD14 glycoprotein, gp 55, is present on most monocytic and macrophages like cell types: monocytes, macrophages, Kupffer cells, pleural phagocytic cells and dendritic reticular cells. CD14 is also observed on granulocytes and activated or transformed B-cells. Furthermore CD14 is present in a soluble form in human serum, urine and other body fluids. The CD14 molecule has been reported to be a receptor for endotoxin.

Application Details

Application Notes: Suitable for Immunostaining of CD14 positive cells (flow cytometry), ELISA, CD14 inhibition studies, Western Blot. 20ug/ml inhibits binding of 0.5 ug/ ml LPS to CD14, binding epitopes amino acid 9-13, 39-44, cross reacting with CD14 of cattle++, swine ++, dog+ horse+ Binding titre at human CD14 transfected CHO-cells (FACS): more than 100.000 Inhibits LPS induced IL1

Concentration: 1 mg/ml

Purification: Protein G purified

Buffer: 0.1mg in PBS without NaN3

Storage: at -20°C

Research Area: Stem Cells, Innate Immunity, Hematopoietic Progenitors, Hematopoietic Stem Cells, CD Antigens, Surface Receptors of Immune Cells, Apoptosis/Necrosis, Immunology, Inflammation

Restrictions: For Research Use only

Publications

Vidal, Labueta, Schiffrin et al.: "Soluble CD14 in human breast milk and its role in innate immune responses." in: Acta odontologica Scandinavica, Vol. 59, Issue 5, pp. 330-4, 2001 (PubMed).

Berner, Fuerll, Stelter et al.: "Elevated levels of lipopolysaccharide-binding protein and soluble CD14 in plasma in neonatal early-onset sepsis." in: Clinical and diagnostic laboratory immunology, Vol. 9, Issue 2, pp. 440-5, 2002 (PubMed).

Kato, Ogasawara, Homma et al.: "Lipopolysaccharide-binding protein critically regulates lipopolysaccharide-induced IFN-beta signaling pathway in human monocytes." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 172, Issue 10, pp. 6185-94, 2004 (PubMed).