CD14 Molecule (CD14) antibody

Details for Product No. ABIN343168
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Antigen
Reactivity
Human
(385), (78), (29), (23), (22), (20), (15), (13), (10), (8), (8), (8), (7), (7), (1), (1)
Host
Mouse
(339), (77), (28), (10), (3)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(58), (39), (37), (21), (14), (4), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Flow Cytometry (FACS), Western Blotting (WB), Functional Studies (Func), ELISA
(303), (112), (101), (50), (49), (45), (39), (29), (25), (15), (10), (6), (3), (3), (2), (2), (1), (1), (1), (1), (1)
Pubmed 5 references available
Quantity 100 µg
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Catalog No. ABIN343168
319.44 $
Plus shipping costs $45.00

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  • +1 404 474 4654
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Immunogen Monocytes of different species and immunoaffinity purified soluble human CD14
Clone BiG 13
Isotype IgG1
Purification Protein G purified
Alternative Name CD14
Background BiG 13 represents an excellent marker for CD14. The CD14 glycoprotein, gp 55, is present on most monocytic and macrophages like cell types: monocytes, macrophages, Kupffer cells, pleural phagocytic cells and dendritic reticular cells. CD14 is also observed on granulocytes and activated or transformed B-cells. Furthermore CD14 is present in a soluble form in human serum, urine and other body fluids. The CD14 molecule has been reported to be a receptor for endotoxin.
Research Area Stem Cells, Innate Immunity, Hematopoietic Progenitors, Hematopoietic Stem Cells, CD Antigens, Surface Receptors of Immune Cells, Apoptosis/Necrosis, Immunology, Inflammation
Application Notes Suitable for Immunostaining of CD14 positive cells (flow cytometry), ELISA, CD14 inhibition studies, Western Blot. 20ug/ml inhibits binding of 0.5 ug/ ml LPS to CD14, binding epitopes amino acid 9-13, 39-44, cross reacting with CD14 of cattle++, swine ++, dog+ horse+ Binding titre at human CD14 transfected CHO-cells (FACS): more than 100.000 Inhibits LPS induced IL1
Restrictions For Research Use only
Format Lyophilized
Concentration 1 mg/ml
Buffer 0.1mg in PBS without NaN3
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Product cited in: Bache, Spreitzer, Becker et al.: "Bordetella Pertussis Toxin does not induce the release of pro-inflammatory cytokines in human whole blood." in: Medical microbiology and immunology, Vol. 201, Issue 3, pp. 327-35, 2012 (PubMed). Further details: Monoclonal anti-CD14 antibody (anti-CD14 mAb, biG14) was used to block the CD14 receptor for LPS

Moyo, Marchi, Steinbach: "Differentiation and activation of equine monocyte-derived dendritic cells is not correlated with CD206 or CD83 expression." in: Immunology, 2013 (PubMed).

General Vidal, Labueta, Schiffrin et al.: "Soluble CD14 in human breast milk and its role in innate immune responses." in: Acta odontologica Scandinavica, Vol. 59, Issue 5, pp. 330-4, 2001 (PubMed).

Berner, Fuerll, Stelter et al.: "Elevated levels of lipopolysaccharide-binding protein and soluble CD14 in plasma in neonatal early-onset sepsis." in: Clinical and diagnostic laboratory immunology, Vol. 9, Issue 2, pp. 440-5, 2002 (PubMed).

Kato, Ogasawara, Homma et al.: "Lipopolysaccharide-binding protein critically regulates lipopolysaccharide-induced IFN-beta signaling pathway in human monocytes." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 172, Issue 10, pp. 6185-94, 2004 (PubMed).

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