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CD14 Molecule (CD14) antibody

Antigen

CD14 Molecule (CD14)
Synonyms CD14
Clonality Monoclonal (BiG 57)
Host
Alternatives

Mouse
Reactivity
Alternatives

Mouse (Murine)
Application
Alternatives Flow Cytometry (FACS), Western Blotting (WB), Functional Studies (Func), ELISA
Catalog no. ABIN343179
Quantity 100ug  (3.4 mg/ml)
Price 325.00 $   Plus shipping costs $35.00
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Additional Information
Application Details Alternatives Secondary Antibodies back to top

Alternative name CD14
Immunogen CD14 knock out mouse immunized with recombinant mouse CD14
Format Lyophilized
Isotype IgG1  (Matching secondary antibodies)
Clone BiG 57
Description BiG 57 represents an excellent marker for mouse CD14. The CD14 glycoprotein, gp 55, is present on most monocytic and macrophages like cell types: monocytes, macrophages, Kupffer cells, pleural phagocytic cells and dendritic reticular cells. CD14 is also observed on granulocytes and activated or transformed B-cells. Furthermore CD14 is present in a soluble form in mouse serum, urine and other body fluids. The CD14 molecule has been reported to be a receptor for endotoxin.

Application Details
Alternatives Secondary Antibodies back to top

Application Notes ELISA , immunostaining of mouse macrophages after stimulation 6h with LPS, Western blot with recombinant mouse CD14. Binding titre at mouse CD14 transfected CHO-cells: more than 100.000 (ELISA, Flow cytometry), no binding at LPS-binding site, endotoxin content less than 0.1ng/ml
Concentration 3.4 mg/ml
Purification Protein G purified
Buffer 5mg without NaN3
Storage long time storage at -20°C or
Research Area Stem Cells, Innate Immunity, Hematopoietic Progenitors, Hematopoietic Stem Cells, CD Antigens, Surface Receptors of Immune Cells, Apoptosis/Necrosis, Immunology, Inflammation
Restrictions For Research Use only

Alternatives
Application Details Secondary Antibodies back to top

Alternatives for antigen "CD14 Molecule (CD14)", type "Antibodies"
Hosts Mouse (240), Rabbit (36), Goat (8), Rat (2), Chicken (1)
Reactivities Human (266), Pig (Porcine) (34), Mouse (Murine) (29), Cow (Bovine) (28), Dog (Canine) (26), Sheep (Ovine) (25), Rabbit (24), Monkey (23), Primates (19), Rat (Rattus) (18), Goat (9), Cat (Feline) (8), Primate (8), Chicken (1), Llama (1)
Applications Flow Cytometry (FACS) (217), Western Blotting (WB) (54), ELISA (36), Immunoprecipitation (IP) (32), Functional Studies (Func) (24), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (24), Immunohistochemistry (Frozen Sections) (IHC (fro)) (22), Immunofluorescence (IF) (20), Enzyme Immunoassay (EIA) (19), Immunohistochemistry (IHC) (16), ELISA (Detection) (5), Blocking Antibody (Inhibition) (3), Immunocytochemistry (ICC) (3), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), Immunoelectron Microscopy (IEM) (1), Radioimmunoassay (RIA) (1)
Conjugates FITC (35), PE (24), Biotin (21), APC (16), RPE (8), Cy5.5 (5), Alexa Fluor 488 (3), Pacific Blue (3), Alexa Fluor 700 (2), Cy5 (2), Cy7,PE (2), PE,Cy5 (2), PE,Cy7 (2), PE,DY590 (2), PE,DY647 (2), SPRD (2), APC,Cy7 (1), Alexa Flour 488 (1), Alexa Fluor 350 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), CF (1), Cy3 (1), Cy7 (1), Gold (1), HRP (1), PE,Cy3 (1), PE,Cy5.5 (1), PE-Dyomics 590 (1), PE-Dyomics 647 (1), PerCP (1), RBITC (1), RPE,Cy5 (1), RPE,Cy5.5 (1)
Epitopes C-Term (2), N-Term (2)