CD34 (CD34) antibody

Antigen: CD34 (CD34)

Clonality: Monoclonal (QBEnd-10)

Host: Mouse

Reactivity: Human, Primates

Application: Flow Cytometry (FACS), Immunoprecipitation (IP), Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro)), Functional Studies (Func), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Catalog no.: ABIN343702

Additional Information

Alternative name: CD34

Immunogen: Human endothelial vesicles

Format: Azide Free

Isotype: IgG1  (Matching secondary antibodies)

Clone: QBEnd-10

Description: CD34 is a highly glycosylated monomeric 111-115 kDa surface protein, which is present onmany stem cell populations. It is a well established stem cell marker, though its expressionon human hematopoietic stem cells is reversible. CD34 probably serves as a surfacereceptor that undergoes receptor-mediated endocytosis and regulates adhesion,differentiation and proliferation of hematopoietic stem cells and other progenitors. CD34expression is likely to represent a specific state of hematopoietic development that mayhave altered adhering properties with expanding and differentiating capabilities in both invitro and in vivo conditions.

Specificity: The antibody QBEnd-10 reacts with Class II epitope on CD34 (Mucosialin), a 110-115 kDamonomeric transmembrane phosphoglycoprotein expressed on hematopoietic progenitorscells and on the most pluripotential stem cells, it is gradually lost on progenitor cells. Thisantibody has been also used as an endothelial marker.HLDA V., WS Code BP BP275HLDA V., WS Code E E038HLDA V., WS Code M MA065HLDA V., WS Code M MR09 This antibody does not react with: Rat, Bovine, Sheep, Dog (Canine).

Application Details

Application Notes: Flow Cytometry: Recommended dilution: 5-10 µg/ml. Immunohistochemistry (paraffin sections) - Recommended dilution: 1-2 µg/ml. Immunohistochemistry (frozen sections). Functional Application: The antibody QBEnd-10 induces homotypic adhesion of leukemic cell line.

Concentration: 1 mg/ml

Purity: > 95% (by SDS-PAGE)

Purification: Purified from hybridoma culture supernatant by protein-A affinity chromatography.

Buffer: Azide free phosphate buffered saline (PBS), approx. pH 7.4, 0.2 µ m filter sterilized.

Storage: Store at 2-8 o C. Do not use after expiration date stamped on vial label. For long-term storage aliquot and store at -20 o C. Avoid freeze/thaw cycles.

Research Area: Hematopoietic Progenitors, Hematopoietic Stem Cells, Cardiogenesis, Lineage Markers, CD Antigens, Surface Receptors of Immune Cells

Restrictions: For Research Use only

Publications

Publications: Kuzu, Bicknell, Harris et al.: "Heterogeneity of vascular endothelial cells with relevance to diagnosis of vascular tumours." in: Journal of clinical pathology, Vol. 45, Issue 2, pp. 143-8, 1992 (PubMed).

Sutherland, Marsh, Davidson et al.: "Differential sensitivity of CD34 epitopes to cleavage by Pasteurella haemolytica glycoprotease: implications for purification of CD34-positive progenitor cells." in: Experimental hematology, Vol. 20, Issue 5, pp. 590-9, 1992 (PubMed).

Schlingemann, Rietveld, de Waal et al.: "Leukocyte antigen CD34 is expressed by a subset of cultured endothelial cells and on endothelial abluminal microprocesses in the tumor stroma." in: Laboratory investigation; a journal of technical methods and pathology, Vol. 62, Issue 6, pp. 690-6, 1990 (PubMed).

Ramani, Bradley, Fletcher: "QBEND/10, a new monoclonal antibody to endothelium: assessment of its diagnostic utility in paraffin sections." in: Histopathology, Vol. 17, Issue 3, pp. 237-42, 1991 (PubMed).

Traorue, Hirn: "Certain anti-CD34 monoclonal antibodies induce homotypic adhesion of leukemic cell lines in a CD18-dependent and a CD18-independent way." in: European journal of immunology, Vol. 24, Issue 10, pp. 2304-11, 1994 (PubMed).

Poblet, Jimenez-Acosta, Rocamora: "QBEND/10 (anti-CD34 antibody) in external root sheath cells and follicular tumors." in: Journal of cutaneous pathology, Vol. 21, Issue 3, pp. 224-8, 1994 (PubMed).

Grimsley, Amos, Gordon et al.: "Rapid positive selection of CD34+ cells using magnetic microspheres coated with monoclonal antibody QBEND/10 linked via a cleavable disulphide bond." in: Leukemia : official journal of the Leukemia Society of America, Leukemia Research Fund, U.K, Vol. 7, Issue 6, pp. 898-908, 1993 (PubMed).