LST1 antibody

Antigen: LST1

Clonality: Monoclonal (LST1-02)

Host: Mouse

Reactivity: Human

Application: Immunoprecipitation (IP), Western Blotting (WB), Immunocytochemistry (ICC)

Catalog no.: ABIN343705

Additional Information

Immunogen: KLH-fused peptide corresponding to amino acids SSEGPDLRGRDKRGT of human LST1

Format: Purified

Isotype: IgG1  (Matching secondary antibodies)

Clone: LST1-02

Description: LST1 (leukocyte-specific transcript, also known as B144) is expressed in cells ofmyeloid/erythroid lineage (monocytes, granulocytes, dendritic cells, platelets, erythrocytes).At least 14 alternatively spliced variants (LST1/A LST1/N) can be detected, some of them(LST1/A, B, C, G, I, K) are transmembrane cell surface-exposed forms, the other aresoluble. LST1 induces production of long, thin filopodia in dendritic cells, has an inhibitoryeffect on lymphocyte proliferation and may have an immunomodulatory role. LST1/A is an11 kDa transmembrane adaptor present in membrane rafts and forms spontaneouslycovalent homodimers. Its intracellular domain contains two tyrosine motifs, one of thembeing an ITIM very similar to such motifs in Siglec.

Specificity: The antibody LST1/02 reacts with an extracellular epitope of LST1, an approximately 6-11kDa protein expressed as various transmembrane or soluble forms. LST1 is foundpredominantly on monocytes and dendritic cells. It migrates on SDS PAGE gels asapproximately 25-28 kDa molecule.

Application Details

Application Notes: Immunoprecipitation - Positive control: RAJI human Burkitt lymphoma cell line. Application note: It migrates on SDS PAGE gels as approximately 25-28 kDa molecule. Immunocytochemistry

Concentration: 1 mg/ml

Purity: > 95% (by SDS-PAGE)

Purification: Purified from ascites by protein-A affinity chromatography.

Buffer: Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4

Storage: Store at 2-8 o C. Do not use after expiration date stamped on vial label. For long-term storage aliquot and store at -20 o C. Avoid freeze/thaw cycles.

Research Area: Neurology, Innate Immunity, Cytokines

Restrictions: For Research Use only

Images

Western Blotting analysis (non-reducing conditions) of LST1 in whole cell lysate of U937 human Caucasian histiocytic lymphoma cell line.Lane 1: immunostaining with Isotype mouse IgG1 control (PPV-06, cat. no. 11-457-C100)Lane 2-3: immunostaining with anti-human LST1 (LST1/02), two independent batches.Note: It migrates on SDS PAGE gels as approximately 25-28 kDa molecule.