AKR1C3 antibody (AA 311-323)

Catalog No. ABIN343889

The Goat Polyclonal anti-AKR1C3 antibody is suitable to detect AKR1C3 in samples from Human. It has been validated for WB, ELISA, IHC and IHC (p).

Quick Overview for AKR1C3 antibody (AA 311-323) (ABIN343889)

Target: AKR1C3 (Aldo-Keto Reductase Family 1, Member C3 (3-alpha Hydroxysteroid Dehydrogenase, Type II) (AKR1C3))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This AKR1C3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-AKR1C3 Antibody

Binding Specificity: AA 311-323

Specificity: Human AKR1C3.

Predicted Reactivity: Percent identity with other species by BLAST analysis: Human (100%), Gorilla (92%), Orangutan (92%), Gibbon (92%), Monkey (92%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide C-FASHPNYPYSDEY from the C-terminus of human AKR1C3 (NP_003730.4). Percent identity with other species by BLAST analysis: Human (100%), Gorilla (92%), Orangutan (92%), Gibbon (92%), Monkey (92%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), Peptide-ELISA (1:32000), WB (1:32000, 0.01 - 0.1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for AKR1C3

Target: AKR1C3 (Aldo-Keto Reductase Family 1, Member C3 (3-alpha Hydroxysteroid Dehydrogenase, Type II) (AKR1C3))

Alternative Name: DDX / AKR1C3

Background: Name/Gene ID: AKR1C3

Synonyms: AKR1C3, 3-alpha-HSD type 2, 17-beta-HSD 5, 3-alpha-HSD type II, brain, DD3, DDH1, DDX, Dihydrodiol dehydrogenase 3, Dihydrodiol dehydrogenase X, DD-3, HA1753, HAKRe, HAKRB, HSD17B5, KIAA0119, Kia0119, Prostaglandin F synthase, PGFS, Truncated aldoketo reductase a, HluPGFS, Indanol dehydrogenase

Gene ID: 8644

NCBI Accession: NP_003730

UniProt: P42330

Pathways: Retinoic Acid Receptor Signaling Pathway, Steroid Hormone Biosynthesis, Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, C21-Steroid Hormone Metabolic Process, Protein targeting to Nucleus