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Phosphothreonine-PROLINE antibody

Antigen

Phosphothreonine-PROLINE

Clonality Monoclonal
Host

Mouse

Application
Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
Catalog no. ABIN344517
Quantity 100 µl
Price 540.00 $   Plus shipping costs $35.00
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Availability Ships within 10 to 15 Business Days

Additional Information

Alternative name Phospho-Threonine-Proline
Immunogen Synthetic peptide, Synthetic phosphothreonine- proline-containing peptides (KLH coupled). Antibody is a mouse IgM clone and can be recognized by anti-mouse Ig (whole molecule) secondary antibody.
Isotype IgM  (Matching secondary antibodies)
Description Gene: Phospho-Threonine-Proline
Specificity Detects phospho- threonine only when followed by proline. It reacts with proteins/peptides phosphorylated on the Thr-Pro motif in an otherwise highly context-independent fashion. The phosphospecific antibody does not recognize phospho-threonine in the absence of an adjacent proline. Does not react with phospho-tyrosine, but does react with some phospho-serine peptides containing the phospho-serine-proline motif (e. g., phospho-Elk-1). Recognizes all species. Predicted Reactivities: Human,Mouse (Murine),Rabbit,Rat (Rattus),Sheep (Ovine).

Application Details

Application Notes ELISA (1:1000), IHC-P, WB (1:5000)
Purification Protein A Column
Buffer 10 mM HEPES, pH 7.5, 150 mM sodium chloride, 0.1 mg/ml BSA, 50% glycerol. No preservative added.
Restrictions For Research Use only