Phosphothreonine-PROLINE antibody

Catalog No. ABIN285914

The Rabbit Polyclonal anti-Phosphothreonine-PROLINE antibody is suitable to detect Phosphothreonine-PROLINE. It has been validated for WB and ELISA.

Quick Overview for Phosphothreonine-PROLINE antibody (ABIN285914)

Target: Phosphothreonine-PROLINE

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB), ELISA

Product Details

Specificity: Recognizes protein containing phosthronine-proline motif, also reacts to phosphoserine-prolin motif to a similar degree. The antibodies do not react to phosphothreonie, phosphoserine or phsophotyrosine under optimal conditions.0.02% NaN3

Immunogen: Phosphothreonine-Pro antibody was raised in rabbit using KLH-phosphothreonine-proline amine (pT-P-NH2) peptide conjugate as the immunogen.

Application Details

Application Notes: ELISA, 0.5 µg/mL, WB: 2 µg/mL
Optimal conditions should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: Lot specific

Buffer: PBS with 0.2 % NaN3.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium Azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: DO NOT FREEZE.
Dilute only prior to immediate use.

Storage: 4 °C

Target Details

Target: Phosphothreonine-PROLINE

Target Type: Chemical

Background: Threonine is one of the 20 naturally occurring amino acids. Kinases phosphorylate threonine to phosphothreonine which enters various biosynthetic pathways in the body. The threonine residue is susceptible to numerous posttranslational modifications. The hydroxy side-chain can undergo O-linked glycosylation. In addition, threonine residues undergo phosphorylation through the action of a threonine kinase. In its phosphorylated form, it can be referred to as phosphothreonine.