Phospho-Threonine-X-Arginine antibody

Antigen: Phospho-Threonine-X-Arginine

Clonality: Polyclonal

Host: Rabbit

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Catalog no.: ABIN344518

Additional Information

Immunogen: Synthetic peptide containing the phospho-Thr-X-Arg motif.

Isotype: IgG  (Matching secondary antibodies)

Description: Gene: Phospho-Threonine-X-Arginine

Specificity: Detects endogenous levels of proteins containing the phospho-Thr-X-Arg motif. Detects phosphorylated Thr followed by Arg or Lys at the +2 position, though its reactivity is lower for the Lys compared to Arg at the +2 position. Does not cross-react with nonphospho-Thr or phospho-Ser in the same motif. It recognizes phospho-Thr in the FFT*R motif in PKCbeta II but does not recognize phospho-Thr in other motifs that lack Lys or Arg at +2. Recognizes all species. Predicted Reactivities: Human,Mouse (Murine),Rabbit,Rat (Rattus),Sheep (Ovine).

Application Details

Application Notes: ELISA (1:1000), IHC-P, WB (1:1000)

Purification: Protein A Column

Buffer: 10 mM HEPES, pH 7.5, 150 mM sodium chloride, 0.1 mg/ml BSA, 50% glycerol. No preservative added.

Restrictions: For Research Use only