Histone 3 antibody (ciArg2, ciArg8, ciArg17)

Catalog No. ABIN4889193

The Mouse Monoclonal anti-Histone 3 antibody (Clone 7C10) (ABIN4889193) specifically detects Histone 3 in IF, ELISA, ICC and FACS. The antibody is reactive with Human samples.

Quick Overview for Histone 3 antibody (ciArg2, ciArg8, ciArg17) (ABIN4889193)

Target: Histone 3 (H3) (Histone H3 (H3))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Histone 3 antibody is un-conjugated

Application: Immunofluorescence (IF), ELISA, Immunocytochemistry (ICC), Flow Cytometry (FACS)

Clone: 7C10

Product Details anti-Histone 3 Antibody

Binding Specificity: ciArg2, ciArg8, ciArg17

Specificity: Reacts with human Histone H3 Citrulline at the 3 positions: 2, 8 and 17

Cross-Reactivity (Details): No cross reaction with mono or bis H3-Cit. Not tested in other species. May cross reacts with other species due to sequence homology.

Purification: Purified (protein A)

Immunogen: Synthetic peptide derived from Nter domain of human Histone H3 containing Citrulline at position 2-8-17

Isotype: IgG1 kappa

Application Details

Application Notes:

Working dilution: Optimal dilution should be determined by the end user.
The following are guidelines only :
ELISA1:20 000 - 1:100 000 ICC/ IF1:100 -1:500

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Must be reconstituted in distilled water.

Concentration: 1 mg/mL

Buffer: Tris 0,1M, glycine 0,1M, sucrose 2 %

Storage: 4 °C/-20 °C

Storage Comment: Lyophilized powder stable for a minimum of 2 years at -20°C. Store reconstituted antibodies at +4°C. For extended periods store in aliquots at -20°C. Antibodies are guaranteed for 6 month from date of receipt.

Expiry Date: 24 months

Target Details for Histone 3

Target: Histone 3 (H3) (Histone H3 (H3))

Alternative Name: Histone H3

Background: Histone H3 is citrullinated by PADI4 this modification induce a repression of transcription and impairs the methylation.

UniProt: Q6NXT2