Peptidyl Arginine Deiminase, Type III (PADI3) antibody

Antigen: Peptidyl Arginine Deiminase, Type III (PADI3)

Synonyms: PAD3, PDI3, MGC126307, MGC126308, Pad3, Pdi3, AI507004, KIAA4171, mKIAA4171, PADI3

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human, Rat (Rattus), Mouse (Murine)

Application: ELISA, Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blotting (WB)

Catalog no.: ABIN347067

Additional Information

Alternative name: Peptidylarginine deiminase type III (PAD-3)

Immunogen: A15 residue synthetic peptide derived from N-terminal domain

Format: Antiserum

Specificity: Antiserum reacts specifically with 75 kDa of PAD3 protein from mouse, human and rat. Cross Reactivities: There is no cross reaction with the other isoforms

Application Details

Application Notes: Optimal dilutions should be determined by the end user.The following are guidelines only: ELISA :1/5 000 to 1/20 000. Immunohistochemistry : 1/50 to 1/500. Immunoprecipitation : 1/20 to 1/100. Western Blot : 1/200 to 1/2 000

Buffer: Lyophilized from 100 µl of serum. Preservative: None.

Storage: Short term storage: +4°C. Long term storage: -20°C

Restrictions: For Research Use only

Publications

Publications: Kanno, Kawada, Yamanouchi et al.: "Human peptidylarginine deiminase type III: molecular cloning and nucleotide sequence of the cDNA, properties of the recombinant enzyme, and immunohistochemical localization in human skin." in: The Journal of investigative dermatology, Vol. 115, Issue 5, pp. 813-23, 2000 (PubMed).

Muechin, Enji, Nachat et al.: "The peptidylarginine deiminases expressed in human epidermis differ in their substrate specificities and subcellular locations." in: Cellular and molecular life sciences : CMLS, Vol. 62, Issue 17, pp. 1984-95, 2005 (PubMed).