PADI3 antibody (N-Term)

Catalog No. ABIN347067

This Rabbit Polyclonal antibody specifically detects PADI3 in WB, ELISA, IHC, IP, IF and ICC. It exhibits reactivity toward Human, Mouse and Rat. It has been mentioned in 3+ publications

Quick Overview for PADI3 antibody (N-Term) (ABIN347067)

Target: PADI3 (Peptidyl Arginine Deiminase, Type III (PADI3))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PADI3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunofluorescence (IF), Immunocytochemistry (ICC)

Product Details anti-PADI3 Antibody

Binding Specificity: N-Term

Specificity: Reacts with 75 kDa PADI3 protein from mouse, human and rat

Cross-Reactivity (Details): No cross reaction with the other isoforms.

Purification: Antiserum

Immunogen: A 15 residue synthetic peptide derived from N-terminal domain of human PADI3 protein

Isotype: IgG

Application Details

Application Notes:

Working dilution: Optimal dilution should be determined by the end user.
The following are guidelines only :
ELISA(1:5000 - 1:20000) IHC / IF(1:50 - 1:500) IP(1:20 - 1:100) WB(1:200 - 1:2000)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Must be reconstituted in distilled water.

Storage: 4 °C/-20 °C

Storage Comment: Lyophilized powder stable for a minimum of 2 years at -20°C. Store reconstituted antibodies at +4°C. For extended periods store in aliquots at -20°C. Antibodies are guaranteed for 6 month from date of receipt.

Expiry Date: 24 months

References for anti-PADI3 antibody (ABIN347067)

Young, Rothfuss, Gard, Muth, Thompson, Ashley, Cherrington: "Citrullination regulates the expression of insulin-like growth factor-binding protein 1 (IGFBP1) in ovine uterine luminal epithelial cells." in: Reproduction (Cambridge, England), Vol. 153, Issue 1, pp. 1-10, (2018) (PubMed).

Khan, Edwards, Muth, Thompson, Cherrington, Navratil: "GnRH stimulates peptidylarginine deiminase catalyzed histone citrullination in gonadotrope cells." in: Molecular endocrinology (Baltimore, Md.), pp. me20161085, (2016) (PubMed).

Li, Hayward, Jenkins, Rothfuss, Young, Nevalainen, Muth, Thompson, Navratil, Cherrington: "Peptidylarginine Deiminase 3 (PAD3) Is Upregulated by Prolactin Stimulation of CID-9 Cells and Expressed in the Lactating Mouse Mammary Gland." in: PLoS ONE, Vol. 11, Issue 1, pp. e0147503, (2016) (PubMed).

Target Details for PADI3

Target: PADI3 (Peptidyl Arginine Deiminase, Type III (PADI3))

Alternative Name: Protein-Arginine Deiminase Type-3

Background: Catalyzes the deimination of arginine residues of proteins. Involved in protein citrullination.

Gene ID: 51702

UniProt: Q9ULW8