Histone H3 / Citrulline 2-8-17 antibody

Antigen: Histone H3 / Citrulline 2-8-17

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Immunohistochemistry (IHC), Western Blotting (WB)

Catalog no.: ABIN347087

Additional Information

Immunogen: Synthetic peptide derived from N terminal region of Human Histone H3, containing Citrulline at position 2,8,17

Format: Antiserum

Specificity: Antiserum reacts specifically with human Histone H3 Citrulline at either position 2 and or 8 and or 17. Cross Reactivities: Not yet tested in other species, but the antibody should react with a wide range of species .Due to sequence homology

Application Details

Application Notes: Optimal dilutions should be determined by the end user. The following are guidelines only: Immunohistochemistry : 1/100 to 1/500. Western Blot : 1/200 to 1/1 000

Buffer: Lyophilized from 100 µl of serum. Preservative: None.

Storage: Short term storage: +4°C. Long term storage: -20°C

Restrictions: For Research Use only

Publications

Publications: Mastronardi, Wood, Mei et al.: "Increased citrullination of histone H3 in multiple sclerosis brain and animal models of demyelination: a role for tumor necrosis factor-induced peptidylarginine deiminase 4 translocation." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 26, Issue 44, pp. 11387-96, 2006 (PubMed).

Raijmakers, Zendman, Egberts et al.: "Methylation of arginine residues interferes with citrullination by peptidylarginine deiminases in vitro." in: Journal of molecular biology, Vol. 367, Issue 4, pp. 1118-29, 2007 (PubMed).