PLCz1 antibody (Internal Region)

Catalog No. ABIN347397

This Rabbit Polyclonal antibody specifically detects PLCz1 in ELISA, WB, IHC and ICC. It exhibits reactivity toward Human and has been mentioned in 2+ publications.

Quick Overview for PLCz1 antibody (Internal Region) (ABIN347397)

Target: PLCz1 (Phospholipase C zeta 1 (PLCz1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PLCz1 antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC)

Product Details anti-PLCz1 Antibody

Binding Specificity: Internal Region

Specificity: Reacts with human PLC zeta protein

Cross-Reactivity (Details): No cross reaction

Purification: Antiserum

Immunogen: Synthetic peptide derived from internal part of PLC-zeta-1

Isotype: IgG

Application Details

Application Notes:

Working dilution: Optimal dilution should be determined by the end user.
The following are guidelines only:
WB(1:100 - 1:1000) ICC(1:50 - 1:1000)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Must be reconstituted in distilled water.

Storage: 4 °C/-20 °C

Storage Comment: Lyophilized powder stable for a minimum of 2 years at -20°C. Store reconstituted antibodies at +4°C. For extended periods store in aliquots at -20°C. Antibodies are guaranteed for 6 month from date of receipt.

Expiry Date: 24 months

References for anti-PLCz1 antibody (ABIN347397)

Janghorban-Laricheh, Ghazavi-Khorasgani, Tavalaee, Zohrabi, Abbasi, Nasr-Esfahani: "An association between sperm PLCζ levels and varicocele?" in: Journal of assisted reproduction and genetics, (2016) (PubMed).

Kamali-Dolat Abadi, Tavalaee, Shahverdi, Nasr-Esfahani: "Evaluation of PLCζ and PAWP Expression in Globozoospermic Individuals." in: Cell journal, Vol. 18, Issue 3, pp. 438-45, (2016) (PubMed).

Target Details for PLCz1

Target: PLCz1 (Phospholipase C zeta 1 (PLCz1))

Alternative Name: Phospholipase C zeta Isoform (PLC-zeta-1)

Background: The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes. In vitro, hydrolyzes PtdIns(4,5)P2 in a Ca2+-dependent manner. Triggers intracellular Ca2+ oscillations in oocytes solely during M phase and is involved in inducing oocyte activation and initiating embryonic development up to the blastocyst stage. Is therefore a strong candidate for the egg-activating soluble sperm factor that is transferred from the sperm into the egg cytoplasm following gamete membrane fusion.

Gene ID: 89869

UniProt: Q86YW0