V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) antibody

Antigen: V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1)

Synonyms: AKT, PKB, RAC, PRKBA, MGC99656, PKB-ALPHA, RAC-ALPHA, Akt, pkb, xAct, akt-1, v-akt, v-akt1, AKT1, AKT/PKB, D-Akt, DAKT1, DAKT1/PKB, DPKB, DRAC-PK, DRAC-PK66; DRAC-PK85, Dakt, PKB/AKT, PKB/dAKT, RacPK, dAKT/dPKB, dAkt/PKB, l(3)04226, l(3)89Bq, DmelCG4006, CG4006

Clonality: Monoclonal (18F10-E5)

Host: Mouse

Reactivity: Human

Application: ELISA, Western Blotting (WB), Immunohistochemistry (IHC)

Catalog no.: ABIN349616

Additional Information

Alternative name: AKT

Gene ID: NP_005154

Swiss-Prot: P31749

Immunogen: This monoclonal antibody was produced by repeated immunizations with a synthetic peptide corresponding to residues near the C terminal end of of human AKT1 protein.

Format: Liquid (sterile filtered)

Isotype: IgG1 kappa  (Matching secondary antibodies)

Clone: 18F10-E5

Description: AKT is a component of the PI-3 kinase pathway and is activated by phosphorylation at Ser 473 and Thr 308. AKT is a cytoplasmic protein also known as AKT1, Protein Kinase B (PKB) and rac (related to A and C kinases). AKT is a key regulator of many signal transduction pathways. AKT exhibits tight control over cell proliferation and cell viability. Overexpression or inappropriate activation of AKT is noted in many types of cancer. AKT mediates many of the downstream events of PI 3-kinase (a lipid kinase activated by growth factors, cytokines and insulin). PI 3-kinase recruits AKT to the membrane, where it is activated by PDK1 phosphorylation. Once phosphorylated, AKT dissociates from the membrane and phosphory-lates targets in the cytoplasm and the cell nucleus. AKT has two main roles: (i) inhibition of apoptosis, (ii) promotion of proliferation.

Specificity: This product was purified from concentrated tissue culture supernate by Protein A chromatography. This antibody is specific for human and mouse AKT protein. A BLAST analysis was used to suggest cross-reactivity with AKT from human, mouse, rat and chimpanzee sources based on 100% homology with the immunizing sequence. Cross-reactivity with AKT from other sources has not been determined. Cross-reactivity with AKT2 and AKT3 has not been determined.

Synonyms: AKT, PKB, RAC, PRKBA, MGC99656, PKB-ALPHA, RAC-ALPHA, Akt, pkb, xAct, akt-1, v-akt, v-akt1, AKT1, AKT/PKB, D-Akt, DAKT1, DAKT1/PKB, DPKB, DRAC-PK, DRAC-PK66; DRAC-PK85, Dakt, PKB/AKT, PKB/dAKT, RacPK, dAKT/dPKB, dAkt/PKB, l(3)04226, l(3)89Bq, DmelCG4006, CG4006

Application Details

Application Notes: Recommended Dilutions: ELISA 1:20,000 WESTERN BLOT 1:500 - 1:3,000 IMMUNOHISTOCHEMISTRY 20 ug/ml OTHER APPLICATIONS User Optimized. Note: This monoclonal antibody is suitable for ELISA, immunohistochemistry, immunoprecipitation and western blotting. Expect a band approximately 56 kDa in size corresponding to AKT protein by western blotting in the appropriate cell lysate or extract. This monoclonal antibody reacts with human and mouse AKT. Specific conditions for reactivity should be optimized by the end user. For immunohistochemistry we recommend the use of fresh frozen tissues. Attempts at staining paraffin-embedded formalin fixed tissues were negative.

Concentration: 1.0 mg/ml (by UV absorbance at 280 nm)

Buffer: Stabilizer: None. Preservative: 0.01% (w/v) Sodium Azide. Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2.

Storage: Store vial at -20o C or below prior to opening. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20o C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of opening.

Research Area: Signaling

Restrictions: For Research Use only

Images

Western blot using Anti-AKT monoclonal antibody shows detection of AKT (indicated by arrowhead at ~56 kDa) on unstimulated (lane 1) and PDGF stimulated NIH/3T3 cell lysates (lane 2). Each lane contained approximately 10 ug of lysate. All samples were loaded on to a 4-20% gradient gel for separation. After electrophoresis, the gel was blocked with 5% BLOTTO (p/n B501-0500 in TBS for 2h at RT. The membrane was probed with the primary antibody at a 1:1,000 dilution in TBS with 5% BLOTTO, overnight at 4o C. For detection HRP conjugated Gt-a-Mouse IgG (p/n 610-103-121) was used at a 1:40,000 dilution for 1 h at 4o C with FemtoMaxTM enhanced chemiluminescent reagent (p/n FEMTOMAX-100). Images were captured using 2X2 binning for 10-20 sec using a BioSpectrum Imaging System (UVP Ltd.) 130 95 72 55 36 28 17 250 1 2